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首页> 外文期刊>Applied Surface Science >Novel Tissue-engineered Vascular Patches Based On Decellularized Canine Aortas And Their Recellularization In Vitro
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Novel Tissue-engineered Vascular Patches Based On Decellularized Canine Aortas And Their Recellularization In Vitro

机译：基于脱细胞犬主动脉的新型组织工程化血管斑块及其体外再细胞化

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摘要

Decellularized allo- or xenogeneic vascular grafts have been found to give more promising results than some biodegradable synthetic polymers. However, owing to absence of well-organized cells, especially confluent endothelial cells, their long-term patency is limited. Seeding vessel-originated cells on these grafts may overcome the deficiency. In this study, canine aortas were decellularized and cross-linked. 4',6-Diamidino-2-phenylindole(DAPI)and Masson' trichrome staining showed complete removal of cell debris, while structure integrity of extracellular matrix (ECM) was remained. Human umbilical vein endothelial cells (HUVECs) and human umbilical artery smooth muscle cells (HUASMCs) were seeded on these decellularized aorta patches in three manners, ECs alone (EC/O), SMCs alone (SMC/O) and ECs on SMCs layer (EC/SMC). In EC/O and SMC/O, scanning electron microscopy (SEM) examination indicated both cells could form confluent layers on the decellularized patches when seeded at high density, but their morphology and alignment changed with seeding density. In EC/SMC, ECs could grow well on SMCs layer, but their morphology, alignment, and confluence degree were deeply influenced by the density of SMCs beneath.
机译：与某些可生物降解的合成聚合物相比，已发现脱细胞的同种异体或异种血管移植物可提供更有希望的结果。然而，由于缺乏组织良好的细胞，特别是融合的内皮细胞，它们的长期开放性受到限制。在这些移植物上播种源自血管的细胞可以克服这一缺陷。在这项研究中，犬主动脉被脱细胞并交联。 4'，6-Diamidino-2-phenylindole（DAPI）和Masson'三色染色显示细胞碎片被完全清除，而细胞外基质（ECM）的结构完整性得以保留。将人脐静脉内皮细胞（HUVEC）和人脐动脉平滑肌细胞（HUASMC）以三种方式分别接种在这些脱细胞的主动脉贴片上，即单独的EC（EC / O），单独的SMC（SMC / O）和在SMCs层上的EC（ EC / SMC）。在EC / O和SMC / O中，扫描电子显微镜（SEM）检查表明，当以高密度接种时，两个细胞都可以在脱细胞贴剂上形成汇合层，但是它们的形态和排列随接种密度而变化。在EC / SMC中，ECs可以在SMCs层上很好地生长，但是它们的形态，排列和汇合程度受下面SMCs的密度影响很大。

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