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首页> 外文期刊>Applied Surface Science >Adhesion And Endothelialization Of Endothelial Cells On The Surface Of Endovascular Stents By The Novel Rotational Culture Of Cells
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Adhesion And Endothelialization Of Endothelial Cells On The Surface Of Endovascular Stents By The Novel Rotational Culture Of Cells

机译：新型细胞旋转培养法在血管支架表面上内皮细胞的粘附和内皮化

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Recent researches indicate that the initial event in the implantation of endovascular stents involves mechanical injury to the vessel wall. Confluent endothelialization of vascular grafts in vitro before implantation has been suggested as a way to reduce injury of the blood vessel. The purpose of this study is to establish a useful way to improve the adhesion of endothelial cells and accelerate endothelialization on the surface of endovascular stents by a novel rotational culture device. Numerical simulation was used to predict the shear stress on the surface of stents. The number of cellular adhesion was calculated by cell counting, the cell growth was observed by scanning electron microscope and fluorescence microscope. Numerical simulation results showed that the stents was exposed to shear stress of 2.66 × 10 ~3 to 8.88 × 10~(-2) Pa. Rotational culture of human umbilical vein endothelial cells could enhance the adhesion of cells and accelerate endothelialization on the surface of stents when the culture conditions for EC adhesion were intermediate rotation speed, higher dynamic incubation times, lower cell densities.
机译：最近的研究表明，血管内支架植入的初始事件涉及对血管壁的机械损伤。已经提出在植入前在体外对血管移植物进行融合内皮化作为减少血管损伤的方法。这项研究的目的是建立一种有用的方法，通过一种新型的旋转培养装置来改善内皮细胞的粘附并加速血管内支架表面的内皮化。数值模拟被用来预测支架表面的剪切应力。通过细胞计数计算细胞粘附数目，通过扫描电子显微镜和荧光显微镜观察细胞生长。数值模拟结果表明，支架承受的切应力为2.66×10〜3至8.88×10〜（-2）Pa。人脐静脉内皮细胞的旋转培养可增强细胞的黏附力并促进内皮表面的内皮化。当EC粘附的培养条件为：中等旋转速度，较高的动态孵育时间，较低的细胞密度时，则需要使用支架。

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