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首页> 外文期刊>Applied Surface Science >Surface Modification Of Polyethylene Terephthalate With Albumin And Gelatin For Improvement Of Anticoagulation And Endothelialization
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Surface Modification Of Polyethylene Terephthalate With Albumin And Gelatin For Improvement Of Anticoagulation And Endothelialization

机译：用白蛋白和明胶对聚对苯二甲酸乙二酯进行表面改性，以改善抗凝和内皮化作用

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The surfaces of polyethylene terephthalate (PET) were modified by oxygen plasma-induced and ultraviolet (UV)-assisted acrylic acid (AAc) grafting polymerization, and the carboxyl (-COOH) groups on the PET surface was 5.29 × 10 ~(-9)mol/cm~2. Then using the -COOH as reacting sites, the molecules of gelatin and bovine serum albumin (BSA) were further co-immobilized on the PET surface. The modified PET surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and surface chemical quantitative analysis. The results showed that the molecules of gelatin and albumin were immobilized on the PET surface. The concentration of gelatin on the gelatin-immobilized PET surface was 2.02 μg/cm~2. For the gelatin-immobilized PET surface, the human umbilical vein endothelial cells (HUVECs) culture attachment and proliferation ratios were improved, but the anticoagulation became worse proved by platelet adhesion test in vitro and the lactate dehydrogense (LDH) test. After further co-immobilization of albumin with gelatin biomolecules on the PET surface (PET-Gel-BSA), the percent of platelet adhesion in vitro decreased 28% than that on the gelatin-immobilized PET surface, and the cell density on the PET-Gel-BSA film (1.08 × 10~5 cells/cm~2) was significantly higher than that on the control PET surface. This investigation tries to find a method which can construct the anticoagulant surface before the endothelium formation and also accelerate the endothelialization of polymer surface.
机译：聚对苯二甲酸乙二醇酯（PET）的表面经过氧等离子体诱导和紫外线（UV）辅助丙烯酸（AAc）接枝聚合反应改性，PET表面的羧基（-COOH）为5.29×10〜（-9摩尔/ cm〜2。然后使用-COOH作为反应位点，将明胶和牛血清白蛋白（BSA）的分子进一步共固定在PET表面。改性的PET表面通过X射线光电子能谱（XPS）和表面化学定量分析进行表征。结果表明，明胶和白蛋白分子被固定在PET表面上。明胶固定的PET表面上的明胶浓度为2.02μg/ cm〜2。对于明胶固定的PET表面，人脐静脉内皮细胞（HUVEC）的培养附着和增殖率得到了改善，但体外血小板粘附试验和乳酸脱氢酶（LDH）试验证明抗凝作用变差。将白蛋白与明胶生物分子进一步共固定在PET表面（PET-Gel-BSA）后，离体血小板粘附的百分比比明胶固定的PET表面降低了28％，PET- Gel-BSA膜（1.08×10〜5细胞/ cm〜2）明显高于对照PET表面。该研究试图找到一种方法，该方法可以在内皮形成之前构建抗凝血表面并且还可以加速聚合物表面的内皮化。

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