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Characterization And Human Gingival Fibroblasts Biocompatibility Of Hydroxyapatite/pmma Nanocomposites For Provisional Dental Implant Restoration

机译:羟基磷灰石/ pmma纳米复合材料的特性及人牙龈成纤维细胞的生物相容性,用于临时性种植体修复

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The aim of this study was to determine nHA/PMMA composites (H/P) in an optimal ratio with improved cytocompatibility as well as valid physical properties for provisional dental implant restoration. 20 wt.%, 30 wt.%, 40 wt.% and 50 wt.% H/P were developed and characterized using XPS, bending strength test and SEM. Human gingival fibroblasts cultured in extracts or directly on sample discs were investigated by fluorescent staining and MTT assay. Chemical integration in nHA/PMMA interface was indicated by XPS. Typical fusiform cells with adhesion spots were detected on H/P discs. MTT results also indicated higher cell viability in 30 wt.% and 40 wt.% H/P discs (P<0.05). We conclude that nHA addition to PMMA enhances cytocompatibility and the optimal nHA/PMMA ratio for provisional fixed crowns (PFC) is 0.4:1.
机译:这项研究的目的是确定最佳比例的nHA / PMMA复合材料(H / P),具有改善的细胞相容性以及临时牙种植体修复的有效物理特性。使用XPS,抗弯强度测试和SEM,开发并表征了20重量%,30重量%,40重量%和50重量%的H / P。通过荧光染色和MTT分析研究了提取物中或直接在样品盘上培养的人牙龈成纤维细胞。 XPS指示nHA / PMMA界面中的化学结合。在H / P光盘上检测到典型的具有粘附点的梭形细胞。 MTT结果还表明在30wt。%和40wt。%的H / P盘中细胞活力更高(P <0.05)。我们得出的结论是,除PMMA以外,nHA还可以增强细胞相容性,临时固定牙冠(PFC)的最佳nHA / PMMA比为0.4:1。

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