• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Surface Science >A Collagen Based Vitro Model Of Angiogenesis Designed For Tissue-engineering Material
【24h】

A Collagen Based Vitro Model Of Angiogenesis Designed For Tissue-engineering Material

机译：专为组织工程材料设计的基于胶原的血管生成体外模型

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Angiogenesis is central importance to tissue-engineering. Many vitro models are developed to study the mechanism of angiogenesis, making a great deal of contribution to drug development against tumor, and often may be expensive, time-consuming. Till now, few reported models have been applied to evaluating the effect of degradation fluid of tissue-engineering material to angiogenesis. In present study, we used ECV304 cell as the model cell line, type I collagen matrix that contained no stimulatory factors as a culture substratum to develop a testing model. Tube-like structure (TLS) formed within 8 h on lower density of collagen (0.2, 0.5 mg/ml), which is not found on dense collagen (1, 2 mg/ml). After ECV304 cells were seeded on the surface of collagen matrix, adherence occurred within 1 h. Soon afterwards, ECV304 cells migrated into cell aggregates, then sent out elongated cell processes to form TLS by cytoplasmic anastomosis. Proliferation was obviously perceived during the course. To investigate the efficiency of the model, we took poly(lactic acid) (PLA) degradation fluid with degradation time varying from 1 to 120 days as the testing material. TLS formation is enhanced by ECV304 cells exposed to early degradation fluid before 50-day point, and the trend of inhibition grew as the degradation time increased. Further, no formation was found in degradation fluid after 90-day point. The model is sensitive to the surrounding environment, and can demonstrate the effects of testing material quantitatively to angiogenesis. In summary, the simplicity, reproducibility and miniaturized character of the model described here may make it highly useful as a medium to test the effect of degradation fluid of tissue-engineering material to angiogenesis.
机译：血管生成对组织工程至关重要。已开发出许多体外模型来研究血管生成的机理，这为抗肿瘤药物开发做出了巨大贡献，而且通常可能是昂贵，费时的。迄今为止，很少有报道的模型用于评估组织工程材料的降解液对血管生成的影响。在本研究中，我们使用ECV304细胞作为模型细胞系，将不含刺激因子的I型胶原蛋白基质作为培养基质来开发测试模型。管状结构（TLS）在8小时内形成于较低密度的胶原蛋白（0.2，0.5 mg / ml）上，而在密集的胶原蛋白（1，2 mg / ml）上则未发现。将ECV304细胞播种在胶原蛋白基质表面后，在1小时内发生粘附。此后不久，ECV304细胞迁移到细胞聚集体中，然后通过细胞质吻合发出延长的细胞过程以形成TLS。在该过程中明显地观察到增殖。为了研究模型的效率，我们以降解时间从1到120天不等的聚乳酸（PLA）降解液作为测试材料。通过在50天前暴露于早期降解液中的ECV304细胞可增强TLS的形成，并且抑制作用随降解时间的增加而增加。此外，在90天后，在降解液中未发现任何形成。该模型对周围环境敏感，可以证明定量测试材料对血管生成的作用。总之，这里描述的模型的简单性，可再现性和微型化特性使其非常有用，可作为测试组织工程材料降解液对血管生成的作用的介质。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号