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首页> 外文期刊>Applied Surface Science >Protein Quantification On Dendrimer-activated Surfaces By Using Time-of-flight Secondary Ion Mass Spectrometry And Principal Component Regression
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Protein Quantification On Dendrimer-activated Surfaces By Using Time-of-flight Secondary Ion Mass Spectrometry And Principal Component Regression

机译：通过飞行时间二次离子质谱和主成分回归分析树枝状大分子活化表面上的蛋白质定量

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摘要

Interaction between streptavidin and biotin on poly(amidoamine) (PAMAM) dendrimer-activated surfaces and on self-assembled monolayers (SAMs) was quantitatively studied by using time-of-flight secondary ion mass spectrometry (ToF-SIMS). The surface protein density was systematically varied as a function of protein concentration and independently quantified using the ellipsometry technique. Principal component analysis (PCA) and principal component regression (PCR) were used to identify a correlation between the intensities of the secondary ion peaks and the surface protein densities. From the ToF-SIMS and ellipsometry results, a good linear correlation of protein density was found. Our study shows that surface protein densities are higher on dendrimer-activated surfaces than on SAMs surfaces due to the spherical property of the dendrimer, and that these surface protein densities can be easily quantified with high sensitivity in a label-free manner by ToF-SIMS.
机译：通过飞行时间二次离子质谱（ToF-SIMS）定量研究了链霉亲和素与生物素在聚（酰胺基胺）（PAMAM）树枝状聚合物活化的表面和自组装单分子膜（SAMs）上的相互作用。表面蛋白的密度随蛋白浓度的变化而系统地变化，并使用椭偏仪进行独立定量。主成分分析（PCA）和主成分回归（PCR）用于确定次级离子峰强度与表面蛋白密度之间的相关性。从ToF-SIMS和椭偏测量结果，发现蛋白质密度具有良好的线性相关性。我们的研究表明，由于树枝状聚合物的球形特性，树枝状聚合物活化的表面上的表面蛋白质密度高于SAMs表面，并且可以通过无标签方式通过ToF-SIMS轻松地以高灵敏度定量这些表面蛋白质密度。 。

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