• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Surface Science >Relative Quantification Of Cellular Sections With Molecular Depth Profiling Tof-sims Imaging
【24h】

Relative Quantification Of Cellular Sections With Molecular Depth Profiling Tof-sims Imaging

机译：分子深度分析Tof-sims成像对细胞切片的相对定量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We report the use of secondary ion mass spectrometry (SIMS) imaging to quantify the relative difference in the amount of lipid between two sections, the plasma membrane and the cytoplasm, of single cells from two different populations. Cells were each labeled with lipophillic dyes, frozen, fractured and analyzed in a ToF-SIMS mass spectrometer equipped with a 40 keV C_(60)~+ ion source. In addition to identifying cells from separate populations, the lipophilic dyes can be used as a marker for the outer leaflet of the cell membrane and therefore as a depth finder. Here, we show that it is possible to compare the amount of lipids with particular headgroups in the cell membrane of a treated cell to the membrane of a control cell. Following erosion of the cell membranes, the amount of the two specific lipid head groups in the cytoplasm of the treated cell can be compared to those lipids in a control cell. Here we take the first step in this experimental design and display the ability to analyze multiple sections of frozen cells following a single fracture.
机译：我们报告了使用二次离子质谱（SIMS）成像来量化两个不同种群的单个细胞的两个部分（质膜和细胞质）之间脂质量的相对差异。每个细胞都用亲脂性染料标记，冷冻，破碎并在配备40keV C_（60）〜+离子源的ToF-SIMS质谱仪中进行分析。除了鉴定来自不同种群的细胞外，亲脂性染料还可以用作细胞膜外部小叶的标记，因此可以用作深度检测器。在这里，我们表明可以比较处理过的细胞的细胞膜与对照细胞的膜中具有特定头基的脂质的量。在细胞膜被侵蚀之后，可以将被处理细胞的细胞质中两个特定脂质头基团的量与对照细胞中的那些脂质进行比较。在这里，我们迈出了这一实验设计的第一步，并展示了在单个断裂后分析冷冻细胞的多个部分的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号