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首页> 外文期刊>Applied Surface Science >Imaging lipid distributions in model monolayers by ToF-SIMS with selectively deuterated components and principal components analysis
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Imaging lipid distributions in model monolayers by ToF-SIMS with selectively deuterated components and principal components analysis

机译：使用选择性氘化成分和主成分分析的ToF-SIMS对模型单层中的脂质分布进行成像

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Time of flight secondary ion mass spectrometry (ToF-SIMS) provides the capability to image the distribution of molecular ions and their associated fragments that are emitted from monolayer films. ToF-SIMS can be applied to the analysis of monolayers of complex lipid mixtures that act as a model to understand the organization of cell membranes into solid-like domains called lipid rafts. The ability to determine the molecular distribution of lipids using ToF-SIMS in monolayer films is also important in studies of the function of pulmonary surfactant. One of the limitations of the use of ToF-SIMS to studies of complex lipid mixtures found in biological systems, arises from the similarity of the mass fragments that are emitted from the components of the lipid mixture. The use of selectively deuterated components in a mixture overcomes this limitation and results in an unambiguous assignment of specific lipids to particular surface domains. The use of deuterium labeling to identify specific lipids in a multicomponent mixture can be done by the deuteration of a single lipid or by the addition of more than one lipid with selectively deuterated components. The incorporation of deuterium into the lipid chains does not alter the miscibility or phase behavior of these systems. The use of deuterium labeling to identify lipids and determine their distribution in monolayer films will be demonstrated using two biological systems. Principal components analysis (PCA) is used to further analyze these deuterated systems checking for the origin of the various mass fragments present. (c) 2006 Elsevier B.V. All rights reserved.
机译：飞行时间二次离子质谱（ToF-SIMS）提供了对单层膜发射的分子离子及其相关碎片的分布进行成像的功能。 ToF-SIMS可用于分析复杂脂质混合物的单分子层，这些模型可作为模型来了解细胞膜组织成称为脂质筏的固体样结构域的模型。在单层薄膜中使用ToF-SIMS确定脂质分子分布的能力在研究肺表面活性剂的功能中也很重要。使用ToF-SIMS来研究生物系统中发现的复杂脂质混合物的局限性之一是由脂质混合物各组分发出的质量碎片的相似性引起的。在混合物中使用选择性氘化的组分克服了该限制，并导致特定脂质明确分配至特定表面结构域。氘标记可用于鉴定多组分混合物中的特定脂质，可通过单个脂质的氘化或通过添加多于一种的具有选择性氘化组分的脂质来完成。氘掺入脂质链不会改变这些系统的混溶性或相行为。使用两种生物系统将证明使用氘标记来识别脂质并确定其在单层膜中的分布。主成分分析（PCA）用于进一步分析这些氘代系统，以检查存在的各种质量碎片的来源。 （c）2006 Elsevier B.V.保留所有权利。

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