• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Surface Science >Quantitative analysis of supported membrane composition using the NanoSIMS
【24h】

Quantitative analysis of supported membrane composition using the NanoSIMS

机译：使用NanoSIMS定量分析支持的膜成分

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have improved methods reported earlier [11 for sample preparation, imaging and quantifying components in supported lipid bilayers using high-resolution secondary ion mass spectrometry performed with the NanoSIMS 50. By selectively incorporating a unique stable isotope into each component of interest, a component-specific image is generated from the location and intensity of the unique secondary ion signals exclusively produced by each molecule. Up to five species can be simultaneously analyzed. Homogeneous supported lipid bilayers that systematically varied in their isotopic enrichment levels were freeze-dried and analyzed with the NanoSIMS 50. The molecule-specific secondary ion signal intensities had an excellent linear correlation to the isotopically labeled lipid content. Statistically indistinguishable calibration curves were obtained using different sample sets analyzed months apart. Fluid bilayers can be patterned using lithographic methods and the composition of each corralled region varied systematically by simple microfluidic methods. The resulting composition variations can be imaged and quantified. This approach opens the possibility of imaging and quantifying the composition of microdomains within membranes, including protein components, without using bulky labels and with very high lateral resolution and sensitivity. (c) 2006 Elsevier B.V. All rights reserved.
机译：我们已经改进了先前报道的方法[11，使用NanoSIMS 50进行高分辨率二次离子质谱分析，对支持的脂质双层中的样品制备，成像和定量。通过将独特的稳定同位素选择性地掺入每个感兴趣的组分中，从每个分子专门产生的独特次级离子信号的位置和强度生成特定图像。最多可以同时分析五种。冷冻干燥并利用NanoSIMS 50分析系统富集的同位素富集水平均一的脂质双分子层。分子特异性次级离子信号强度与同位素标记的脂质含量具有极好的线性相关性。使用相隔数月分析的不同样本集获得了统计上无法区分的校准曲线。可以使用光刻方法对流体双层进行构图，并通过简单的微流体方法系统地改变每个腐蚀区域的组成。由此产生的成分变化可以成像和量化。这种方法打开了成像和定量化膜中微区（包括蛋白质成分）组成的可能性，而无需使用笨重的标记并且具有非常高的横向分辨率和灵敏度。 （c）2006 Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号