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首页> 外文期刊>Applied Surface Science >Frequency shift feedback imaging in liquid for biological molecules
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Frequency shift feedback imaging in liquid for biological molecules

机译：液体中生物分子的频移反馈成像

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A commercially available atomic force microscope (AFM) equipped with a hand made simple self-oscillation circuit was used in imaging biomolecular samples in liquid environments, i.e. under physiological conditions. Assembled tau proteins, which are the major component of the neurofibrillary deposits in Alzheimer's disease, was taken as a trial sample. In order to image its native structure, the protein was physically absorbed on a cleaved mica surface without fixation. Using the frequency feedback imaging with a self-oscillation technique, the structure of protein fibers was clearly imaged even in a wide scanning range (3.75 mum) with a contact force less than 100 pN. Furthermore, no damage of the proteins was observed in successive imagings. This indicates that the deformation of proteins was negligible in our method. In contrast, the proteins were destroyed when the vertical applied force of above 300 pN was applied using the amplitude feedback imaging with the self-oscillation technique. (C) 2002 Elsevier Science B.V. All rights reserved. [References: 16]
机译：配备有手工制作的简单自激振荡电路的市售原子力显微镜（AFM）用于在液体环境中（即在生理条件下）对生物分子样品进行成像。组装的tau蛋白是试验性样品，它是阿尔茨海默氏病神经纤维沉积的主要成分。为了成像其天然结构，该蛋白质在不固定的情况下物理吸附在裂解的云母表面上。使用具有自激振荡技术的频率反馈成像技术，即使在接触力小于100 pN的宽扫描范围（3.75微米）中，也可以清晰地成像蛋白质纤维的结构。此外，在连续成像中未观察到蛋白质的破坏。这表明在我们的方法中蛋白质的变形可以忽略不计。相反，当使用具有自激振荡技术的振幅反馈成像施加300 pN以上的垂直施加力时，蛋白质被破坏。 （C）2002 Elsevier Science B.V.保留所有权利。 [参考：16]

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