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首页> 外文期刊>Applied Surface Science >Drosophila melanogaster as an in vivo model to study the potential toxicity of cerium oxide nanoparticles
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Drosophila melanogaster as an in vivo model to study the potential toxicity of cerium oxide nanoparticles

机译：果蝇果蝇作为体内模型研究氧化铈纳米粒子的潜在毒性

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Cerium oxide nanoparticles (nCeO(2)) were synthesized via the hydroxide mediated approach using cerium nitrate hexahydrate and sodium hydroxide as precursors. The nanoparticle was characterized using TEM, XRD and FTIR analysis. Its toxicity at 0.1 mM and 1 mM doses was investigated using wild-type third instar larvae and adult male flies (Oregon K strain) exposed to nCeO(2) along with food for one month. Ascorbic acid (17 mM) was used as a positive control. Results from the survival assay revealed that when compared to control, both 0.1 mM and 1 mM doses were non-toxic for a period of one month, after which the 1 mM dose was more toxic than the 0.1 mM dose. Dietary administration of nCeO(2) at both the doses did not cause developmental and behavioral defects. Important biochemical parameters such as ROS generation, superoxide dismutase activity, carbohydrate and protein levels, and acetylcholinesterase activity were not significantly altered at both the doses of the nanoparticle, when compared to control. The mRNA expression of antioxidant enzymes such as catalase and superoxide dismutase also was not significantly altered in the third instar larvae that fed on nanoparticle-containing food. The results from the above studies suggest that nCeO(2) at 0.1 mM and 1 mM doses did not elicit any significant toxicity in third instar larvae and adult flies when administered for a period of one month. This study also suggests that nCeO(2) can be used as a potential anti-oxidant compound and a beneficial drug carrier in Drosophila disease models in future studies.
机译：通过使用六水合硝酸铈和氢氧化钠作为前体的氢氧化物介导的方法合成了氧化铈纳米颗粒（nCeO（2））。使用TEM，XRD和FTIR分析表征纳米颗粒。使用野生型三龄幼虫和暴露于nCeO（2）并与食物接触一个月的成年雄性果蝇（俄勒冈州K株）研究了其在0.1 mM和1 mM剂量下的毒性。抗坏血酸（17 mM）用作阳性对照。存活试验的结果表明，与对照组相比，0.1 mM和1 mM剂量在一个月的时间内均无毒，此后1 mM剂量比0.1 mM剂量毒性更大。两种剂量的nCeO（2）饮食管理都不会引起发育和行为缺陷。与对照相比，在两个纳米剂量下，重要的生化参数（如ROS生成，超氧化物歧化酶活性，碳水化合物和蛋白质水平以及乙酰胆碱酯酶活性）均未显着改变。在以含纳米颗粒的食物为食的第三龄幼虫中，抗氧化酶如过氧化氢酶和超氧化物歧化酶的mRNA表达也没有明显改变。从上述研究的结果表明，nCeO（2）在0.1 mM和1 mM剂量下给药一个月的时间不会对第三龄幼虫和成年果蝇引起任何明显的毒性作用。这项研究还表明，在未来的研究中，nCeO（2）可用作果蝇疾病模型中的潜在抗氧化剂和有益药物载体。

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