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首页> 外文期刊>Applied Surface Science >Surface functionalization of graphene oxide by disodium guanosine 5'-monophosphate and its excellent performance for lipase immobilization
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Surface functionalization of graphene oxide by disodium guanosine 5'-monophosphate and its excellent performance for lipase immobilization

机译：鸟苷5'-单磷酸二钠对氧化石墨烯的表面官能化及其对脂肪酶的固定作用

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摘要

In order to solve the toxic and hazardous issues of the agents in the chemical reduction process of graphene oxide (GO), disodium guanosine 5'-monophosphate (GMP-2Na) was used as an ideal reducing agent for the synthesis of functionalized GO (FGO) for lipase immobilization. At the condition of low amount adsorption of GMP-2Na indicated by the N1s spectra of X-ray photoelectron spectroscopy (XPS), the C/O atomic ratio of the FGO increased from 2.09 to 3.12, indicating the reduction of GO. As the amount of GMP-2Na increased, the intensity of the characteristic peak of GO in the XRD pattern decreased, and the I-D/I-G peak intensity of Raman spectra increased during the functionalization process. It was found that when the concentration of GMP-2Na is 0.20 mg/mL, the degree of hydrophobicity of FGO is conducive to the formation of optimal conformation of lipase. After immobilization, the activity of the immobilized enzyme reaches twice the value of free enzyme. At the same time, the protein loading reaches to 600 mg/g. The immobilized enzyme showed enhanced durability since no obvious decrease was observed after incubation for 8 h at pH 6.0 and pH 7.0. After storage for 35 days, the activity of GO-G@ enzyme was 54.47% higher than that of free enzyme. GO reduced by GMP-2Na provides a possibility to obtain a highly efficient lipase immobilization carrier.
机译：为了解决氧化石墨烯（GO）的化学还原过程中试剂的毒性和有害问题，鸟苷5'-单磷酸二钠（GMP-2Na）被用作合成功能化GO（FGO）的理想还原剂）用于脂肪酶固定化。 X射线光电子能谱（XPS）的N1s光谱表明，在少量GMP-2Na吸附的条件下，FGO的C / O原子比从2.09增加到3.12，表明GO减少。随着GMP-2Na含量的增加，在功能化过程中，X射线衍射图谱中GO特征峰的强度降低，拉曼光谱的I-D / I-G峰强度增加。发现当GMP-2Na的浓度为0.20mg / mL时，FGO的疏水度有利于脂肪酶的最佳构象的形成。固定后，固定化酶的活性达到游离酶值的两倍。同时，蛋白质负载量达到600 mg / g。固定化酶显示出增强的耐久性，因为在pH 6.0和pH 7.0下孵育8小时后未观察到明显的降低。储存35天后，GO-G @酶的活性比游离酶高54.47％。由GMP-2Na还原的GO提供了获得高效脂肪酶固定载体的可能性。

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