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首页> 外文期刊>Applied Surface Science >Graphene biosensor as affinity biosensors for biorecognition between Guanine riboswitch and ligand
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Graphene biosensor as affinity biosensors for biorecognition between Guanine riboswitch and ligand

机译：石墨烯生物传感器作为鸟嘌呤核糖开关与配体之间生物识别的亲和生物传感器

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摘要

Guanine riboswitch (GR) is located in the untranslated region of mRNA that performs important biological functions by interactions with specific ligand molecules. The present standard methods for detecting mRNA has been widely used, but suffer from extra labeling steps, high cost, low throughput, low sensitivities and limited dynamic ranges. The field effect transistor (FET) biosensors showed high performance in detecting a large number of biological analytes. However, the detection of analytes with low charge and small molecule still remains a challenge. In this paper, a label-free graphene field effect transistor (G-FET) biosensor has been developed to detect the binding interactions between GR and four purine analog ligand molecules (GUA, 6GU, 2BP, XAN). We demonstrated that the G-FET biosensor were highly sensitive in detecting the electric-neutral and low molecular weight ligands by using GR as probe. The sensing mechanisms for electric-neutral molecule is revealed by molecular dynamics simulation. The equilibrium dissociation constants K-D and binding free energy Delta G of GR-GUA, GR-6GU, GR-2BP and GR-XAN were obtained by the G-FET biosensor, and the results are comparable with the calculated by molecular dynamics simulation and previous reports. We demonstrated that the G-FET biosensor can be used as affinity biosensors to quantify biomolecular interactions.
机译：鸟嘌呤核糖开关（GR）位于mRNA的非翻译区域，该区域通过与特定配体分子的相互作用而发挥重要的生物学功能。用于检测mRNA的当前标准方法已被广泛使用，但是存在额外的标记步骤，成本高，通量低，灵敏度低和动态范围有限的问题。场效应晶体管（FET）生物传感器在检测大量生物分析物中表现出高性能。然而，低电荷和小分子分析物的检测仍然是一个挑战。本文开发了一种无标记的石墨烯场效应晶体管（G-FET）生物传感器，用于检测GR和四个嘌呤类似物配体分子（GUA，6GU，2BP，XAN）之间的结合相互作用。我们证明了G-FET生物传感器通过使用GR作为探针在检测电中性和低分子量配体方面高度灵敏。通过分子动力学模拟揭示了电中性分子的感应机制。通过G-FET生物传感器获得了GR-GUA，GR-6GU，GR-2BP和GR-XAN的平衡解离常数KD和结合自由能Delta G，其结果与分子动力学模拟和先前的计算结果相当报告。我们证明了G-FET生物传感器可以用作亲和力生物传感器来量化生物分子相互作用。

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