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首页> 外文期刊>Aquatic Sciences - Research Across Boundaries >Exposing local adaptation: synergistic stressors elicit population-specific lactate dehydrogenase-B (ldh-b) expression profiles in Australian barramundi, Lates calcarifer
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Exposing local adaptation: synergistic stressors elicit population-specific lactate dehydrogenase-B (ldh-b) expression profiles in Australian barramundi, Lates calcarifer

机译：暴露局部适应性：协同应激源引起澳大利亚澳洲肺鱼，乳鳞鱼中的种群特异性乳酸脱氢酶-B（ldh-b）表达谱

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摘要

The molecular response of fish to independently and/or concurrently applied ecological stressors (e.g. thermal and/or aerobic stress) can be quantified at the level of transcript abundance (i.e. gene expression). In temperate fish, the expression of the metabolic candidate gene lactate dehydrogenase-B (ldh-b) responds to both aerobic swimming challenge and extended acclimation to various ecologically relevant temperatures. We examined hepatic ldh-b expression in juvenile Lates calcarifer from two geographically, genetically and thermally distinct Australian populations to determine if similar environmental stressors also influence the transcription of this locus in a tropical fish. Hepatic ldh-b expression was quantified following 28-day acclimation to ecologically relevant temperatures (20, 25, 30 and 35°C). Expression was also quantified in L. calcarifer subjected to aerobic swimming challenge at these temperatures. Fish from southern (high latitude) and northern (low latitude) populations within this species’ Australian distribution exhibited a significant increase in hepatic ldh-b expression following aerobic swimming challenge at native temperatures of 25 and 30°C, respectively (p < 0.001). Southern and northern fish also exhibited significant increase in hepatic ldh-b expression (p < 0.001 and p < 0.01, respectively) following 28-day acclimation to heat-stress (35°C). However, only southern fish exhibited significant increase in expression (p < 0.001) following 28-day acclimation to cold-stress (20°C). The novel evidence presented herein suggests that (a) transcription of hepatic ldh-b is responsive to both aerobic and thermal stress when applied independently, and (b) southern Australian L. calcarifer populations may be locally adapted to cooler seasonal water temperatures.
机译：鱼类对独立和/或同时施加的生态胁迫（例如热胁迫和/或需氧胁迫）的分子反应可以在转录本丰富度（即基因表达）水平上定量。在温带鱼类中，代谢候选基因乳酸脱氢酶-B（ldh-b）的表达既响应有氧游泳挑战，又适应各种生态相关温度。我们检查了来自两个地理，遗传和热学上不同的澳大利亚种群的幼年晚生鳞翅目幼虫的肝脏ldh-b表达，以确定是否类似的环境应激源也影响热带鱼中该基因座的转录。在适应与生态相关的温度（20、25、30和35°C）28天后，对肝脏ldh-b的表达进行了定量。还对在这些温度下进行有氧游泳攻击的钙化乳杆菌中的表达进行了定量。该物种在澳大利亚分布中的南部（高纬度）和北部（低纬度）种群的鱼分别在25和30°C的自然温度下进行有氧游泳挑战后，肝脏ldh-b表达显着增加（p <0.001） 。在适应热应激（35°C）28天后，南部和北部鱼类的肝脏ldh-b表达也显着增加（分别为p <0.001和p <0.01）。然而，在适应寒冷胁迫（20°C）28天后，只有南部鱼类的表达显着增加（p <0.001）。本文提供的新颖证据表明，（a）肝ldh-b的转录在单独应用时对有氧和热应激均具有响应，并且（b）澳大利亚南部的鳞翅目种群可局部适应较凉的季节性水温。

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