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Hydrogen isotope discrimination in aquatic primary producers: implications for aquatic food web studies

机译:水生初级生产者中的氢同位素歧视:对水生食物网研究的启示

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Large differences in δ~2H of primary producers between aquatic and terrestrial ecosystems are used to identify subsidies, discriminate organic matter sources, and reduce uncertainty in food web studies. Previous investigations of hydrogen isotope ratios suggest there may be predictable differences between the δ~2H of water and organic matter for different types of primary producers. We define the difference in the net isotopic discrimination between water and bulk organic matter (om) as: Δ_H = (δ~2H_(om) - δ~2H_(water)) ÷ (1 + δ~2H_(water) ÷ 1,000). We summarized Δ_H values from published literature and we measured the δ~2H of water and primary producers in order to compare Δ_H among aquatic and terrestrial primary producers. Measurements were made from three water body types (lake, river, coastal lagoon) and their associated watersheds. Although we predicted a large and equivalent net isotopic discrimination for aquatic primary producers, we found considerable variability among groups of aquatic producers. Macroalgae, benthic microalgae, and phytoplankton had more negative Δ_H values (i.e. greater isotopic discrimination) than both aquatic macrophytes and terrestrial vegetation. The more positive δ~2H_(om) and hence lower Δ_H of terrestrial vegetation was expected due to relative increases in the heavier isotope, deuterium, during transpiration. However, the more positive values of δ~2H_(om) and relatively low Δ_H in aquatic macrophytes, even submerged species, was unexpected. Marine macroalgae had high variability in δ~2H_(om) as a group, but low variability within distinct species. Variability among types of primary producers in δ~2H_(om) and in Δ_H should be assessed when hydrogen is used in isotopic studies of food webs.
机译:水生生态系统和陆地生态系统之间初级生产者的δ〜2H差异较大,可用于确定补贴,区分有机物来源并减少食物网研究的不确定性。先前对氢同位素比率的研究表明,对于不同类型的初级生产者,水和有机物的δ〜2H之间可能存在可预测的差异。我们定义净水与大块有机物(om)之间的同位素区分的差异为:Δ_H=(δ〜2H_(om)-δ〜2H_(水))÷(1 +δ〜2H_(水)÷1,000) 。我们总结了已发表文献中的Δ_H值,并测量了水和初级生产者的δ〜2H,以便比较水生和陆地初级生产者中的Δ_H。根据三种水体类型(湖泊,河流,沿海泻湖)及其相关流域进行了测量。尽管我们预测了水生初级生产者的净同位素歧视将相当大,但我们发现水生生产者群体之间存在相当大的差异。大型藻类,底栖微藻类和浮游植物比水生大型植物和陆生植被具有更大的负Δ_H值(即更大的同位素分辨力)。由于蒸腾过程中较重的同位素氘相对增加,预计陆地植被的δ〜2H_(om)正值较高,因此Δ_H较低。然而,在水生大型植物,甚至是淹没物种中,δ〜2H_(om)的正值较高,而Δ_H相对较低,这是出乎意料的。海洋大型藻类的δ〜2H_(om)整体具有较高的变异性,但不同物种内部的变异性较低。在食物网的同位素研究中使用氢时,应评估δ〜2H_(om)和Δ_H中初级生产者类型之间的差异。

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