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首页> 外文期刊>Aquatic Sciences >Contribution of deep dark fixation processes to overall CO_2 incorporation and large vertical changes of microbial populations in stratified karstic lakes
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Contribution of deep dark fixation processes to overall CO_2 incorporation and large vertical changes of microbial populations in stratified karstic lakes

机译：深层固色过程对分层喀斯特湖中CO_2的整体吸收和微生物种群垂直变化的贡献

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摘要

We carried out a detailed study in five stratified lakes in the karstic regions of NE Spain along a redox gradient combining vertical profiles of inorganic carbon dioxide fixation and analysis of microbial (bacteria and archaea) community composition determined by 16S rRNA gene fingerprinting (DGGE), microscopic counts, and pigment analysis. High rates of non-photosynthetic (i.e., "dark") inorganic carbon incorporation were detected mostly at deeper layers after short-term in situ incubations at noon. Significant contribution of dark CO_2 incorporation was observed at the whole lake level for the single time sampling, ranging between 4 and 19% of total carbon fixation measured, and up to 31 % in the case of a meromictic basin. Good agreement was found between vertical patterns in redox conditions and the different microbial diversity descriptors (DGGE band sequencing, microscopic analysis, and pigment data), showing large vertical changes in microbial community composition covering a wide range of phylogenetic diversity. Cyanobacteria, Alpha and Beta-Proteobacteria, Actinobacteria, Flavobacteria and Flectobacillaceae were the most frequently recovered groups in the DGGE from oxygenated water masses. In anoxic waters, we found Beta-Proteobacteria mostly of the Rhodoferax group, Gamma-Proteobacteria (Chromatiaceae), Delta-Proteobacteria related to different sulfate reducing bacteria, Chlorobiaceae, and anaerobic Bacteroidetes spread among the Bacteroidales, Flavobacteriales and Saprospiraceae. However, as a whole, we did not find any significant correlation between dark fixation rates and either nutrient distribution and microbial community composition in the study lakes. All of this suggests that (1) different physiologies and ecologies are simultaneously contributing to the process (2) more sensitive methods are needed and more specific compounds measured and (3) some of the non-specialist microbial populations detected may carry out carbon dioxide assimilation in the dark under in situ conditions.
机译：我们沿着氧化还原梯度在西班牙东北部岩溶区的五个分层湖泊中进行了详细研究，结合无机二氧化碳固定的垂直剖面和通过16S rRNA基因指纹图谱（DGGE）确定的微生物（细菌和古细菌）群落组成的分析，显微计数和色素分析。在中午短期原位孵育后，大部分在较深的层中检测到较高的非光合（即“深色”）无机碳掺入率。一次取样在整个湖面上观察到暗CO_2的掺入有显着贡献，范围为所测总固碳量的4％至19％，而在莫莫克性盆地则高达31％。在氧化还原条件下的垂直模式与不同的微生物多样性描述符（DGGE带测序，显微分析和色素数据）之间发现了很好的一致性，表明微生物群落组成的垂直变化很大，涵盖了广泛的系统发育多样性。蓝藻细菌，α和β变形杆菌，放线菌，黄杆菌和丝杆菌科是DGGE中从含氧水团中最常回收的基团。在缺氧水域中，我们发现Beta变形杆菌主要是Rhodoferax组，γ变形杆菌（Chromatiaceae），Delta变形杆菌与与不同硫酸盐还原细菌，绿藻科和厌氧的拟杆菌共生的细菌分布在细菌科，黄杆菌科和腐生菌科中。但是，总体而言，我们在研究湖泊中的固色率与养分分布和微生物群落组成之间没有发现任何显着相关性。所有这些表明（1）不同的生理学和生态学同时促进了这一过程（2）需要更灵敏的方法并测量更具体的化合物，（3）检测到的一些非专业微生物种群可能会进行二氧化碳同化在原地条件下处于黑暗中。

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  • 来源
    《Aquatic Sciences》 |2012年第1期|p.61-75|共15页
  • 作者

    Emilio O. Casamayor; Marc Lliros; Antonio Picazo; Albert Barberan; Carles M. Borrego; Antonio Camacho;

  • 作者单位

    Department of Continental Ecology-Biogeodynamics and Biodiversity Interactions, Centre d'Estudis Avancats de Blanes, CEAB-CSIC, Blanes, Spain;

    Group of Molecular Microbial Ecology, Institut d'Ecologia Aquatica, University of Girona, Girona, Spain,Laboratory of Freshwater Ecology-URBO, Faculte Universitaires Notre Dame de la Paix, University of Namur, Namur, Belgium;

    Department of Microbiology and Ecology, Instituto Cavanilles de Biodiversidad y Biologfa Evolutiva, University of Valencia, Burjassot, Spain;

    Department of Continental Ecology-Biogeodynamics and Biodiversity Interactions, Centre d'Estudis Avancats de Blanes, CEAB-CSIC, Blanes, Spain;

    Group of Molecular Microbial Ecology, Institut d'Ecologia Aquatica, University of Girona, Girona, Spain;

    Department of Microbiology and Ecology, Instituto Cavanilles de Biodiversidad y Biologfa Evolutiva, University of Valencia, Burjassot, Spain;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    primary production; dark carbon fixation; photosynthetic sulfur bacteria; bacteria; archaea; karstic lakes; 16S rRNA; DGGE;

    机译：初级生产;暗碳固色;光合硫细菌;菌;古细菌岩溶湖;16S rRNA;DGGE;

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