...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Aquatic Sciences >Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina
【24h】

Induction of thymine dimers by solar radiation in natural freshwater phytoplankton assemblages in Patagonia, Argentina

机译：阿根廷巴塔哥尼亚天然淡水浮游植物组合中太阳辐射诱导的胸腺嘧啶二聚体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Natural phytoplankton assemblages from a freshwater lake in Trelew ( Province of Chubut, Patagonia, Argentina) were exposed to natural solar radiation at different depths in a water basin filled with fresh water rich in humic substances. Samples were taken at regular intervals for DNA extraction and subsequent analysis of DNA damage by determining the formation of thymine dimers using an immuno-dot-blot procedure. The use of a colorimetric detection system based on the reaction of alkaline phosphatase with BCIP (5-bromo-4-chloro-3-indolyl phosphate)/ NBT ( nitro blue tetrazolium) proved unsatisfactory because of uneven and strong background signals. As a consequence the antibodies were stripped and the blots were reprobed successfully, using a chemoluminescence based detection system. Natural solar radiation caused an increase from 3.2 +/- 0.9 thymine dimers ((TT)-T-boolean AND) per mega base pair (Mbp) to 50.9 +/- 3.8 (TT)-T-boolean AND Mbp(-1) during the light period in natural phytoplankton exposed at the surface. When the UV-B component of the solar radiation was removed by a 320 nm cut-off filter, no thymine dimers were produced. After prolonged exposure at the surface, some but not total recovery occurred overnight. There was no significant DNA damage in the samples exposed at 85 cm depth (k(d) = 3.62 m(-1) at 305 nm). The results indicate that the impinging UV-B radiation at the study site may be deleterious to the survival of phytoplankton exposed at the water surface and thus significantly alter the nutrient cycling and biomass production.
机译：来自Trelew（阿根廷巴塔哥尼亚丘布特省）的淡水湖的天然浮游植物组合在充满了富含腐殖质的淡水的水盆中暴露于不同深度的自然太阳辐射。定期采集样品进行DNA提取，然后通过使用免疫斑点印迹法确定胸腺嘧啶二聚体的形成来分析DNA损伤。基于碱性磷酸酶与BCIP（5-溴-4-氯-3-吲哚磷酸酯）/ NBT（硝基蓝四唑鎓）反应的比色检测系统的使用由于其背景信号不均匀和强而被证明不令人满意。结果，使用基于化学发光的检测系统剥离了抗体并成功地对探针进行了重新探测。天然太阳辐射导致每兆碱基对（Mbp）的胸腺嘧啶二聚体（（+/-）-T-布尔型AND）从3.2 +/- 0.9增加到（Tbp）-T-布尔型AND Mbp（-1）从50.9 +/- 3.8在光照期间，自然浮游植物暴露在水面。当通过320 nm截止滤光片除去太阳辐射的UV-B成分时，没有产生胸腺嘧啶二聚体。长时间暴露在表面后，在一夜之间发生了一些但并非全部的恢复。在暴露于85 cm深度的样品中没有明显的DNA损伤（在305 nm处k（d）= 3.62 m（-1））。结果表明，撞击在研究地点的UV-B辐射可能对暴露于水面的浮游植物的生存有害，从而显着改变了养分循环和生物量的产生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号