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首页> 外文期刊>Arabian Journal for Science and Engineering. Section A, Sciences >Biodegradation of Petrochemical Hydrocarbons Using an Efficient Bacterial Consortium: A2457
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Biodegradation of Petrochemical Hydrocarbons Using an Efficient Bacterial Consortium: A2457

机译：利用高效细菌联盟的石化烃生物降解：A2457

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摘要

Petrochemical hydrocarbons are considered to be the most significant environmental pollutants and need to be removed. In present study, the biodegradation of used engine oil and diesel oil was achieved under shake flask conditions using an efficient bacterial consortium A2457: comprising bacterial strains of Stenotrophomonas maltophilia, Bacillus cereus and Bacillus pumilus. The strains were isolated and screened by enrichment technique. The bacterial strains were identified on the basis of 16S rRNA gene sequence homology. The percent removal and biodegradation of the petrochemicals was evaluated through UV-Vis spectrophotometer and FTIR spectrometry. The bacterial consortium resulted in significant degradation of diesel oil (94.13%) and used engine oil (99.77%) under experimental conditions. FTIR spectra of the hydrocarbons, before and after biodegradation experiments, also revealed significant changes in the characteristic peaks in the wavenumber range of 4000-600cm~(−1). The consortium displayed significant biosurfactant production and lipase activity during 28 days of incubation and reduced the surface tension of culture medium to 27.95 ± 0.3mNm~(−1). The petrochemical hydrocarbon degradation by the bacterial consortium: A2457 of present study could be attributed with the production of biosurfactants and lipases to utilize diesel oil and used engine oil as sole source of carbon and energy.
机译：石油化学烃被认为是最重要的环境污染物，需要被移除。在目前的研究中，使用有效的细菌联盟A2457在摇瓶条件下实现了使用的发动机油和柴油的生物降解：包括牙科霉菌，芽孢杆菌和芽孢杆菌的细菌菌株。通过富集技术分离和筛选菌株。基于16S rRNA基因序列同源性鉴定细菌菌株。通过UV-Vis分光光度计和FTIR光谱法评估石油化学物质的去除率和生物降解的百分比。细菌联盟在实验条件下导致柴油（94.13％）和二手发动机油（99.77％）的显着降解。生物降解实验之前和之后的烃的FTIR光谱还显示出4000-600cm〜（-1）的波数范围内的特征峰的显着变化。该联盟在孵育28天期间显示出显着的生物活性剂生产和脂肪酶活性，并将培养基的表面张力降低至27.95±0.3mnm〜（-1）。细菌联盟的石油化学烃降解：本研究的A2457可归因于生物表面活性剂和脂肪酶的生产，以利用柴油和二手发动机油作为碳和能量的唯一来源。

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