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Isolation of Plant Growth-Promoting Bacillus cereus from Soil and Its Use as a Microbial Inoculant

机译:从土壤中分离植物生长芽孢杆菌及其用作微生物孕育剂

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摘要

Modernization has introduced intensive agricultural practices wherein the pesticides play an important role both in stabilizationand in increase of agricultural products. As a consequence, humans and members of other ecosystems are exposed to increasedlevels of compounds that have detrimental effects on their health, thereby signifying the importance of microbial inoculants. Inorder to achieve this goal 7 different bacterial species were initially screened for isolation of plant growth-promoting Bacillussp. The isolate CUAMS116 was confirmed to be Bacillus cereus through biochemical and molecular characterization. Thein vitro plant growth-promoting ability of the isolatewas screened through standard tests. Different concentrations of bacterialinoculant (25%, 50%, 75%, 100%) were evaluated for its plant growth promotion ability using Phaseolus vulgaris L., underpot culture conditions. At the harvest stage, the mature control plants measured 16.53 cm and mean treated plant height wasmeasured to be 27.75 cm, showing a maximum percentage increase in length of 67.87%. The results suggested that the B.cereus CUAMS116 isolated in this study can be extended as a PGPM through further field trials in other plants for improvingcrop yield and tolerance to biotic and abiotic stresses.
机译:现代化引入了密集的农业实践,其中农药在稳定中发挥着重要作用并增加农产品。结果,人类和其他生态系统的成员都会被暴露于增加对其健康产生不利影响的化合物水平,从而致力于微生物接种剂的重要性。在为了实现这一目标,最初筛选出7种不同的细菌种类以分离植物生长促进芽孢杆菌sp。通过生化和分子表征确认分离物Cuams116是芽孢杆菌。这通过标准测试筛选分离液的体外植物生长能力。不同浓度的细菌使用Puestolus Vulgaris L.的植物生长促进能力评价孕育剂(25%,50%,75%,100%),下盆栽培养条件。在收获阶段,成熟的对照植物测量了16.53cm,平均处理的植物高度是测量为27.75厘米,显示最大百分比的长度为67.87%。结果表明B。本研究中分离的培养CUAMS116可以通过其他植物中的进一步田间试验来延伸为PGPM以改善作物产量和对生物和非生物胁迫的耐受性。

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