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Sensitivity to Urea Fertilization in Three Amphibian Species

机译:三种两栖动物对尿素施肥的敏感性

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Forest fertilization with granular urea is a well-established management practice in many forested regions of the world. We hypothesize that chemical forest fertilizers may be affecting forest-dwelling wildlife. In the laboratory, we studied the effects of fertilization doses of granular urea on three species of forest-dwelling amphibians (Plethodon vehiculum, Rhyacotriton variegatus, and Taricha granulosa). In avoidance experiments, the three species avoided a substrate treated with a dose of 225 kg N/ha urea. In toxicity experiments, we exposed amphibians to urea at doses of 225 kg N/ha and 450 kg N/ha for 4 days. The observed effects increased with time and dose, and there were significant differences in sensitivity among the species. Both treatment levels had an acute effect on survival of P. vehiculum and R. variegatus. At 24 h, mortality at the highest dose was 67% for P. vehiculum, and 47% for R. variegatus. In contrast, there was no mortality for T. granulosa at these concentrations. We suggest that environmental levels of urea could be affecting behavior and survival of some amphibians species in fertilized forests.
机译:在世界上许多森林地区,使用颗粒尿素进行森林施肥是一种行之有效的管理实践。我们假设化学森林肥料可能会影响居住在森林中的野生生物。在实验室中,我们研究了施肥剂量的颗粒尿素对三种居住于森林的两栖动物(Plethodon vehiculum,Rhyacotriton variegatus和Taricha granulosa)的影响。在回避实验中,这三个物种避免了用225 kg N / ha尿素处理的底物。在毒性实验中,我们将两栖动物以225 kg N / ha和450 kg N / ha的剂量暴露于尿素中4天。观察到的效果随着时间和剂量的增加而增加,并且物种之间的敏感性存在显着差异。两种治疗水平对P. vehiculum和R. variegatus的存活都有急性影响。在24 h时,最高剂量的死球菌对P. vehiculum的死亡率为47%,而对R. variegatus则为47%。相比之下,在这些浓度下没有粒状T.的死亡。我们建议环境水平的尿素可能会影响施肥森林中某些两栖动物的行为和生存。

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    Estación Biológica de Doñana Consejo Superior de Investigaciones Cientificas Apartado 1056 Sevilla E-41080 Spain;

    Department of Zoology Oregon State University Corvallis Oregon 97331 USA;

    Department of Zoology Oregon State University Corvallis Oregon 97331 USA;

    Department of Zoology Oregon State University Corvallis Oregon 97331 USA;

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