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首页> 外文期刊>Archives of Environmental Contamination and Toxicology >A Bioassay for the Measurement of Insecticide Concentration
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A Bioassay for the Measurement of Insecticide Concentration

机译:用于测定杀虫剂浓度的生物测定

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A bioassay was developed to measure insecticide residues using fruit flies (Drosophila melongaster). After adding a known volume of sampling solution, the time at which 50% of the flies were dead (LT50) was recorded and cross-referenced to the appropriate calibration curve. Using known standards, comparable results were obtained using the bioassay and GC-MS. The bioassay allows concentrations of synthetic pyrethroids as low as 1 pg L−1 to be measured with a variance of < 5%. The bioassay can be used reliably over a wide range of temperatures and it is tolerant to a range of pH and surface tensions of the test solution. The whole bioassay is compact, physically robust, and simple to use; hence, it could be of use in the field as a quick preliminary assessment of water contamination.
机译:开发了一种生物测定法,以使用果蝇(果蝇(Drosophila melongaster))测量杀虫剂残留。加入已知体积的采样溶液后,记录50%的苍蝇死亡的时间(LT50 ),并将其与适当的校准曲线交叉引用。使用已知的标准品,通过生物测定和GC-MS获得了可比的结果。该生物测定法可测量合成的拟除虫菊酯的浓度低至1 pg L-1 ,方差<5%。该生物测定法可以在很宽的温度范围内可靠地使用,并且可以耐受一定范围的pH值和测试溶液的表面张力。整个生物测定法紧凑,物理坚固且易于使用;因此,它可以在现场用作对水污染的快速初步评估。

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