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首页> 外文期刊>Archives of Pharmacal Research >Quantitative determination of major phlorotannins in Ecklonia stolonifera
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Quantitative determination of major phlorotannins in Ecklonia stolonifera

机译：定量测定Ecklonia stolonifera中主要phlorotannins的含量

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摘要

Ecklonia stolonifera is a rich source of phlorotannins, which are responsible for the potent pharmacological effects associated with this seaweed. The purpose of this study was to develop a reversed-phase high-performance liquid chromatography method for the simultaneous determination of three major phlorotannins, eckol, dieckol, and phlorofucofuroeckol-A, in the extracts of Ecklonia stolonifera. The optimal chromatographic conditions were achieved on a Thermo Hypersil Gold C-18 column (250 × 4.6 mm i.d., 5 μm) using linear gradient elution of acetonitrile and water containing 0.1% formic acid at UV 254 nm. The separated phlorotannins were identified by liquid chromatography-mass spectrometry. The high-performance liquid chromatography method showed good linearity (r2 > 0.998), precision (1.4–9.5%), and accuracy (93.9–108.7%). The limits of detection ranged from 0.06 to 0.30 μg/mL and the lower limits of quantitation ranged from 0.2 to 1.0 μg/mL. Among phlorotannins, dieckol was the most abundant in both ethanol and ethyl acetate extracts of Ecklonia stolonifera.
机译：Ecklonia stolonifera是叶绿单宁的丰富来源，其与这种海藻相关的强效药理作用负责。这项研究的目的是开发一种反相高效液相色谱法，该方法可同时测定菜提取物中的三种主要的邻苯二酚类化合物，eckol，dieckol和phlorofucofuroeckol-A。最佳色谱条件是在Thermo Hypersil Gold C-18色谱柱（250×4.6 mm内径，5μm）上实现的，使用乙腈和含有0.1％甲酸的水在UV 254 nm处进行线性梯度洗脱。通过液相色谱-质谱法鉴定分离的邻苯二酚单宁。高效液相色谱法显示出良好的线性（r2> 0.998），精密度（1.4–9.5％）和准确度（93.9–108.7％）。检测限为0.06至0.30μg/ mL，定量下限为0.2至1.0μg/ mL。在phrotrotannins中，地衣酚在Ecklonia stolonifera的乙醇和乙酸乙酯提取物中均含量最高。

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