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首页> 外文期刊>Archives of Toxicology >Cytotoxicity of peptide-coated silver nanoparticles on the human intestinal cell line Caco-2
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Cytotoxicity of peptide-coated silver nanoparticles on the human intestinal cell line Caco-2

机译：肽包覆的银纳米颗粒对人肠道细胞Caco-2的细胞毒性

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Silver nanoparticles are used in a wide range of consumer products such as clothing, cosmetics, household goods, articles of daily use and pesticides. Moreover, the use of a nanoscaled silver hydrosol has been requested in the European Union for even nutritional purposes. However, despite the wide applications of silver nanoparticles, there is a lack of information concerning their impact on human health. In order to investigate the effects of silver nanoparticles on human intestinal cells, we used the Caco-2 cell line and peptide-coated silver nanoparticles with defined colloidal, structural and interfacial properties. The particles display core diameter of 20 and 40 nm and were coated with the small peptide l-cysteine l-lysine l-lysine. Cell viability and proliferation were measured using Promegas CellTiter-Blue® Cell Viability assay, DAPI staining and impedance measurements. Apoptosis was determined by Annexin-V/7AAD staining and FACS analysis, membrane damage with Promegas LDH assay and reactive oxygen species by dichlorofluorescein assay. Exposure of proliferating Caco-2 cells to silver nanoparticle induced decreasing adherence capacity and cytotoxicity, whereby the formation of reactive oxygen species could be the mode of action. The effects were dependent on particle size (20, 40 nm), doses (5–100 μg/mL) and time of incubation (4–48 h). Apoptosis or membrane damage was not detected.
机译：银纳米颗粒可用于各种消费产品，例如服装，化妆品，家庭用品，日用品和杀虫剂。此外，在欧洲联盟中甚至出于营养目的已经要求使用纳米级银水溶胶。然而，尽管银纳米颗粒得到了广泛的应用，但缺乏有关其对人类健康影响的信息。为了研究银纳米颗粒对人肠细胞的影响，我们使用了具有确定的胶体，结构和界面特性的Caco-2细胞系和肽包覆的银纳米颗粒。颗粒显示出20和40nm的核心直径，并用小肽1-半胱氨酸-赖氨酸-赖氨酸包被。使用Promegas CellTiter-Blue ®细胞活力测定，DAPI染色和阻抗测量来测量细胞活力和增殖。通过膜联蛋白-V / 7AAD染色和FACS分析确定细胞凋亡，通过Promegas LDH分析确定膜损伤，并通过二氯荧光素分析确定活性氧。增殖的Caco-2细胞暴露于银纳米颗粒会导致粘附能力和细胞毒性降低，从而形成活性氧可能是其作用方式。效果取决于粒径（20、40 nm），剂量（5–100μg/ mL）和孵育时间（4–48 h）。未检测到细胞凋亡或膜损伤。

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