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首页> 外文期刊>Archives of Toxicology >Evaluation of the cytotoxic and antimutagenic effects of biflorin, an antitumor 1,4 o-naphthoquinone isolated from Capraria biflora L
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Evaluation of the cytotoxic and antimutagenic effects of biflorin, an antitumor 1,4 o-naphthoquinone isolated from Capraria biflora L

机译：评价比弗林的细胞毒性和抗突变作用，比弗林是一种从Capraria biflora L分离得到的抗肿瘤的1,4 o-萘醌。

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摘要

Biflorin is a natural quinone isolated from Capraria biflora L. Previous studies demonstrated that biflorin inhibits in vitro and in vivo tumor cell growth and presents potent antioxidant activity. In this paper, we report concentration-dependent cytotoxic, genotoxic, antimutagenic, and protective effects of biflorin on Salmonella tiphymurium, yeast Saccharomyces cerevisiae, and V79 mammalian cells, using different approaches. In the Salmonella/microsome assay, biflorin was not mutagenic to TA97a TA98, TA100, and TA102 strains. However, biflorin was able to induce cytotoxicity in haploid S. cerevisiae cells in stationary and exponential phase growth. In diploid yeast cells, biflorin did not induce significant mutagenic and recombinogenic effects at the employed concentration range. In addition, the pre-treatment with biflorin prevented the mutagenic and recombinogenic events induced by hydrogen peroxide (H2O2) in S. cerevisiae. In V79 mammalian cells, biflorin was cytotoxic at higher concentrations. Moreover, at low concentrations biflorin pre-treatment protected against H2O2-induced oxidative damage by reducing lipid peroxidation and DNA damage as evaluated by normal and modified comet assay using DNA glycosylases. Our results suggest that biflorin cellular effects are concentration dependent. At lower concentrations, biflorin has significant antioxidant and protective effects against the cytotoxicity, genotoxicity, mutagenicity, and intracellular lipid peroxidation induced by H2O2 in yeast and mammalian cells, which can be attributed to its hydroxyl radical-scavenging property. However, at higher concentrations, biflorin is cytotoxic and genotoxic.
机译：Biflorin是从Capraria biflora L分离的天然醌。先前的研究表明Biflorin抑制体外和体内肿瘤细胞的生长，并具有强大的抗氧化活性。在本文中，我们报告了使用不同的方法，比弗洛林对沙门氏菌，啤酒酵母和V79哺乳动物细胞的浓度依赖性细胞毒性，基因毒性，抗诱变作用和保护作用。在沙门氏菌/微粒体测定中，双弗洛林对TA97a TA98，TA100和TA102菌株没有致突变性。然而，双弗洛林能够在稳定和指数期生长的单倍体酿酒酵母细胞中诱导细胞毒性。在二倍体酵母细胞中，双florin在所采用的浓度范围内不会诱导明显的诱变和重组作用。此外，用双花青素预处理可以防止酿酒酵母中过氧化氢（H 2 O 2 ）诱导的诱变和重组事件。在V79哺乳动物细胞中，比弗洛林在较高浓度下具有细胞毒性。此外，在低浓度下，比弗洛林预处理可通过减少脂质过氧化作用和DNA损伤来防止H 2 O 2 诱导的氧化损伤，如使用DNA进行正常和改良彗星试验所评估的那样糖基化酶。我们的结果表明，双弗洛林的细胞作用是浓度依赖性的。在较低的浓度下，双弗洛林对酵母和哺乳动物细胞中H 2 O 2 诱导的细胞毒性，遗传毒性，诱变性和细胞内脂质过氧化具有明显的抗氧化和保护作用。可以归因于其清除羟自由基的性质。然而，在较高的浓度下，双弗洛林具有细胞毒性和遗传毒性。

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