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首页> 外文期刊>Archives of Toxicology >Extrapulmonary translocation of intratracheally instilled fine and ultrafine particles via direct and alveolar macrophage-associated routes
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Extrapulmonary translocation of intratracheally instilled fine and ultrafine particles via direct and alveolar macrophage-associated routes

机译：经直接和肺泡巨噬细胞相关途径气管内滴注的细小颗粒和超细颗粒的肺外移位

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Translocation of inhaled ultrafine particles from the lungs into the blood may impair cardiovascular function. We administered ultrafine (20-nm) and fine (200-nm) gold colloid or fluorescein-labeled polystyrene particles to mice intratracheally and examined their localization in the lung and extrapulmonary organs. Fifteen minutes after instillation, dispersed and agglomerated 20-nm gold colloid particles were observed on the surface of endothelial cells, on the alveolar surface, in endocytotic vesicles of alveolar epithelial cells, and in the basement membrane of the lung. A small but noteworthy amount of gold was detected in the liver, kidney, spleen, and heart by inductively coupled plasma-mass spectrometry. After administration of 20- or 200-nm fluorescent particles, free particles were detected infrequently in blood vessels, on the endocardial surface, and in the kidney and liver only in the mice that received 20-nm particles, whereas phagocytes containing 20- or 200-nm particles were found in the extrapulmonary tissues. Fluorescent particle-laden alveolar macrophages administered intratracheally translocated from alveoli to extrapulmonary organs via the blood circulation. Thus, small amounts of ultrafine particles are transported across the alveolar wall into the blood circulation via endocytotic pathways, but particle-laden alveolar macrophages translocate both ultrafine and fine particles from the lungs to the extrapulmonary organs.
机译：吸入的超细颗粒从肺部转移到血液中可能会损害心血管功能。我们向小鼠气管内施用了超细（20 nm）和细（200 nm）金胶体或荧光素标记的聚苯乙烯颗粒，并检查了它们在肺和肺外器官中的定位。滴注后十五分钟，在内皮细胞表面，肺泡表面，肺泡上皮细胞的内吞囊泡以及肺基底膜中观察到分散和团聚的20 nm金胶体颗粒。通过电感耦合等离子体质谱法在肝脏，肾脏，脾脏和心脏中检测到少量但值得注意的金。施用20或200 nm荧光颗粒后，仅在接受20 nm颗粒的小鼠中血管，心内膜表面以及肾脏和肝脏中很少检测到游离颗粒，而吞噬细胞含有20或200 nm在肺外组织中发现1nm的颗粒。气管内给药的载有荧光颗粒的肺泡巨噬细胞通过血液循环从肺泡转移到肺外器官。因此，少量的超细颗粒通过细胞内吞途径穿过肺泡壁进入血液循环，但是充满颗粒的肺泡巨噬细胞同时将超细颗粒和细颗粒从肺转运到肺外器官。

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