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首页> 外文期刊>Archives of Toxicology >A new in vitro cellular system for the analysis of mineral fiber biopersistence
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A new in vitro cellular system for the analysis of mineral fiber biopersistence

机译：一种用于分析矿物纤维生物持久性的新型体外细胞系统

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The toxicity of mineral fibers, whether they are natural or man made (MMMF), is usually evaluated in vivo using biopersistence tests in rodents. Development of an in vitro cellular model would be worthwhile in order to reduce, refine and finally replace animal models. For this purpose, we developed an in vitro assay using human monocytic cell line (U-937) to evaluate a new manufactured rock wool fiber (HDN) biodegradation. Experiments on earlier known mineral fibers asbestos (crocidolite) and glass wool fibers (CM44) were also performed. U-937 responded to HDN and CM44 only if they were activated. Among the different activators we used, Escherichia coli living cells as well as FS were the most efficient as evidenced by alterations of HDN and CM44 surface, detected by scanning electron microscopy, and by the measure of silicon released from the rock wool fibers. Asbestos fibers were not degraded when incubated in the presence of living bacteria. The MMMF modifications were function of the fiber composition, the time of exposure to activated cells and the concentration of activators. The pattern of MMMF degradation by our in vitro system was in accordance with those observed in an in vivo study, thus indicating that the fiber degradation by macrophage cells activated by E. coli living cells as well as FS is a valuable system to assess mineral fibers’ biopersistence.
机译：矿物纤维的毒性，无论是天然纤维还是人造纤维（MMMF），通常都在啮齿动物中使用生物持久性测试在体内进行评估。为了减少，完善并最终替代动物模型，体外细胞模型的开发将是值得的。为此，我们开发了一种利用人单核细胞系（U-937）进行体外测定的方法，以评估新制造的岩棉纤维（HDN）的生物降解能力。还对较早已知的矿物纤维石棉（青石棉）和玻璃棉纤维（CM44）进行了实验。 U-937仅在被激活时才对HDN和CM44做出响应。在我们使用的各种激活剂中，大肠杆菌活细胞和FS效率最高，这可以通过扫描电子显微镜检测到的HDN和CM44表面变化以及岩棉纤维释放的硅含量来证明。在活细菌存在下孵育时，石棉纤维不会降解。 MMMF改性取决于纤维成分，暴露于活化细胞的时间和活化剂的浓度。我们的体外系统对MMMF降解的模式与体内研究中观察到的模式一致，因此表明大肠杆菌活细胞和FS激活的巨噬细胞降解纤维是评估矿物质纤维的有价值的系统生物持久性。

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