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首页> 外文期刊>Arthritis & Rheumatism >Cartilage tissue enhances proteoglycan retention by nucleus pulposus cells in vitro
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Cartilage tissue enhances proteoglycan retention by nucleus pulposus cells in vitro

机译：软骨组织增强髓核细胞在体外的蛋白聚糖保留

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ObjectiveTo investigate the effect of cartilage on nucleus pulposus (NP) tissue in an in vitro model.MethodsCells were isolated from bovine NP or articular cartilage and allowed to form tissue in vitro. The NP tissue was grown either alone or in the presence of cartilage tissue (coculture) for up to 4 weeks and examined for histologic appearance, gene expression, and biochemical composition. For selected experiments, NP tissue was grown in coculture with fragments of cartilage end-plate.ResultsCoculture of in vitro–formed NP tissue with cartilage end-plate tissue resulted in a significant increase in proteoglycan content in the NP tissue by 2 weeks, compared with NP tissue grown alone. Substituting in vitro–formed cartilage tissue for cartilage end-plate also had a positive effect on the NP tissue, suggesting that it was an appropriate substitute for cartilage end-plate. Coculture of NP with in vitro–formed cartilage for 2 weeks increased aggrecan and collagen gene expression compared with that in NP tissue grown alone, and also reduced expression of matrix metalloproteinase 3 (MMP-3), MMP-13, and ADAMTS-5. NP cells from older and younger animals responded similarly to in vitro–formed cartilage. Expression of genes for tumor necrosis factor (TNF) and TACE in NP cells was higher when grown in the absence of cartilage. This corresponded with increased TNF protein levels in the absence of cartilage.ConclusionThe data suggest that chondrocytes may secrete a factor(s) that positively enhances tissue growth, perhaps by inhibiting TNF production. This could be a potential mechanism explaining how loss of the cartilage end-plate may contribute to the development of NP degenerative changes.
机译：目的在体外模型中研究软骨对髓核（NP）组织的影响。方法从牛NP或关节软骨中分离细胞，使其在体外形成组织。 NP组织可以单独生长，也可以在存在软骨组织（共培养）的情况下生长长达4周，并检查其组织学外观，基因表达和生化组成。在选定的实验中，将NP组织与软骨终板片段共培养。结果与2相比，体外形成的NP组织与软骨终板组织共培养导致NP组织中蛋白聚糖含量显着增加了2周。 NP组织单独生长。用体外形成的软骨组织代替软骨终板也对NP组织有积极作用，这表明它是软骨终板的合适替代品。与单独生长的NP组织相比，NP与体外形成的软骨共培养2周可提高软骨聚集蛋白聚糖和胶原基因的表达，并且还可降低基质金属蛋白酶3（MMP-3），MMP-13和ADAMTS-5的表达。来自年老和年幼动物的NP细胞对体外形成的软骨的反应相似。在没有软骨的情况下生长时，NP细胞中的肿瘤坏死因子（TNF）和TACE基因的表达较高。这与在没有软骨的情况下TNF蛋白水平升高相对应。结论数据表明，软骨细胞可能分泌可能通过抑制TNF产生而积极增强组织生长的因子。这可能是解释软骨终板丢失可能如何促进NP退行性改变的潜在机制。

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