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首页> 外文期刊>Asian Journal of Andrology >Expression of a novel pyridoxal kinase mRNA splice variant, PKH-T, in human testis
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Expression of a novel pyridoxal kinase mRNA splice variant, PKH-T, in human testis

机译：新型吡ido醛激酶mRNA剪接变体PKH-T在人睾丸中的表达

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Aim: To identify the genes specifically expressed in human adult and fetal testes and spermatozoa. Methods: A human testis cDNA microarray was established. Then mRNAs of human adult and fetal testis and spermatozoa were purified and probes were prepared by a reverse transcription reaction with mRNA as the template. The microarray was hybridized with probes of adult and fetal testes and spermatozoa. The nucleic acid sequences of differentially expressed genes were determined and homologies were searched in the databases of GenBank. Results: A novel human testis-specific gene, PKH-T, was identified by hybridizing adult and fetal testis and spermatozoa probes with a human testis cDNA microarray. The cDNA of PKH-T was 1 069 bp in length. The cDNA sequence of this clone was deposited in the Genbank (AY303972) and PKH-T was also determined as Interim GenSymbol (Unigene, HS.38041). PKH-T contained most PKH conserved motif. The 239 amino acid sequences deduced from the 719 bp open reading frame (ORF) had a homology with the gene PKH (U89606). PKH-T was specifically and strongly expressed in the testis. Comparison of the differential expressions of PKH and PKH-T in testes of different developmental stages indicated that PKH-T was expressed in the adult testis and spermatozoa, while PKH, in the adult, fetal and aged testes. PKH-T had no expression in the testis of Sertoli cell only and partially spermatogenic arrest patients. Conclusion: PKH-T is a gene highly expressed in adult human testis and spermatozoa. It may play an important role in spermatogenesis and could be related to male infertility.
机译：目的：鉴定在成人，胎儿睾丸和精子中特异性表达的基因。方法：建立人睾丸cDNA微阵列。然后纯化成人和胎儿睾丸及精子的mRNA，并以mRNA为模板，通过反转录反应制备探针。将微阵列与成年和胎儿睾丸以及精子的探针杂交。确定差异表达基因的核酸序列，并在GenBank数据库中搜索同源性。结果：通过将成年和胎儿睾丸和精子探针与人类睾丸cDNA微阵列杂交，鉴定了一种新的人类睾丸特异性基因PKH-T。 PKH-T的cDNA长度为1 069 bp。该克隆的cDNA序列存放在Genbank（AY303972）中，PKH-T也被确定为Interim GenSymbol（Unigene，HS.38041）。 PKH-T包含大多数PKH保守基序。从719 bp开放阅读框（ORF）推导的239个氨基酸序列与PKH（U89606）基因具有同源性。 PKH-T在睾丸中特异性表达并强烈表达。比较不同发育阶段睾丸中PKH和PKH-T的差异表达表明，PKH-T在成人睾丸和精子中表达，而PKH在成人，胎儿和老年睾丸中表达。 PKH-T仅在睾丸支持细胞和部分生精停止的睾丸中不表达。结论：PKH-T是在成人睾丸和精子中高表达的基因。它可能在精子发生中起重要作用，并且可能与男性不育有关。

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