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首页> 外文期刊>Asian Journal of Andrology >Comparative study on efficacy of three sperm-separation techniques
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Comparative study on efficacy of three sperm-separation techniques

机译：三种精子分离技术功效的比较研究

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To evaluate the comparative effectiveness of real-time sperm separation technique (Wang's tube method) and other two conventional methods in isolating high-quality sperm preparation, and to compare the sperm pregnancy rate in intrauterine insemination (IUI) with sperm preparations isolated by these methods. Methods: The effectiveness of the real-time sperm separation technique, the conventional swim-up and the Percoll discontinuous density gradient methods in isolating sperm preparations from 60 infertile patients (20 with apparently normal semen and 40, abnormal semen contaminated with microorganisms and other impurities) was evaluated and compared. The microorganisms to be removed included bacteria, virus, Chlamydia trachomaticum, Ureaplsama urealyticum, etc. The microorganisms to be removed included bacteria, virus, Chamydia trachomaticum, Ureaplsama urealyticum, etc. The spouse pregnancy rates in IUI with sperm preparations isolated by these three techniques from 80 oligoasthenoteratospermic patients were also compared. Results: The quality (including the percentages of normal form, normal-chromatin and motile sperm, and the grade of motility) of sperm obtained by t he real-time sperm separation technique was much higher (P<0.01) as compared with those by the other two methods. The Wang's tube method was also more effective in removing microorganism and other impurities. The method provided a higher IUI pregnancy rate than the other two sperm separation techniques (P<0.05). Conclusion: The real-time sperm separation technique is the most effective method so far availabl
机译：评估实时精子分离技术（Wang's管法）和其他两种常规方法在分离高质量精子制剂中的比较效果，并比较宫内人工授精（IUI）中精子的怀孕率与通过这些方法分离的精子制剂的比较。方法：实时精子分离技术，常规游泳法和Percoll不连续密度梯度法从60例不育患者（20例精液正常，40例精液异常，被微生物和其他杂质污染）中分离精子制剂的有效性）进行了评估和比较。要去除的微生物包括细菌，病毒，沙眼衣原体，解脲脲原体等。要去除的微生物包括细菌，病毒，沙眼衣原体，解脲脲原体等。通过这三种技术分离的精子制剂在IUI中的配偶怀孕率还比较了80例少精子囊性精子症患者。结果：通过实时精子分离技术获得的精子质量（包括正常形式，正常染色质和活动精子的百分比以及活力等级）比经精子分离技术获得的精子质量高（P <0.01）。其他两种方法。王氏管法在去除微生物和其他杂质方面也更有效。该方法提供的IUI妊娠率高于其他两种精子分离技术（P <0.05）。结论：实时精子分离技术是迄今为止最有效的方法。

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