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首页> 外文期刊>Astrobiology >Dried Biofilms of Desert Strains of Chroococcidiopsis Survived Prolonged Exposure to Space and Mars-like Conditions in Low Earth Orbit
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Dried Biofilms of Desert Strains of Chroococcidiopsis Survived Prolonged Exposure to Space and Mars-like Conditions in Low Earth Orbit

机译：沙漠球菌的干燥生物膜在低地球轨道上幸存于长时间暴露于太空和类似火星的条件下

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摘要

Dried biofilms and dried multilayered planktonic counterparts obtained from three desert strains of Chroococcidiopsis were exposed to low Earth conditions by using the EXPOSE-R2 facility outside the International Space Station. During the space mission, samples in Tray 1 (space vacuum and solar radiation, from lambda approximate to 110 nm) and Tray 2 (Mars-like UV flux, lambda 200 nm and Mars-like atmosphere) received total UV (200-400 nm) fluences of about 4.58 x 10(2) kJ/m(2) and 4.92 x 10(2) kJ/m(2), respectively, and 0.5 Gy of cosmic ionizing radiation. Postflight analyses were performed on 2.5-year-old samples due to the space mission duration, from launch to sample return to the lab. The occurrence of survivors was determined by evaluating cell division upon rehydration and damage to the genome and photosynthetic apparatus by polymerase chain reaction-stop assays and confocal laser scanning microscopy. Biofilms recovered better than their planktonic counterparts, accumulating less damage not only when exposed to UV radiation under space and Mars-like conditions but also when exposed in dark conditions to low Earth conditions and laboratory control conditions. This suggests that, despite the shielding provided by top-cell layers being sufficient for a certain degree of survival of the multilayered planktonic samples, the enhanced survival of biofilms was due to the presence of abundant extracellular polymeric substances and to additional features acquired upon drying.
机译：通过使用国际空间站外的EXPOSE-R2设施，将从三种沙漠球菌的沙漠菌株获得的干燥生物膜和干燥的多层浮游生物暴露在低地球条件下。在太空任务期间，托盘1（太空真空和太阳辐射，λ接近110 nm）和托盘2（类似火星的UV通量，λ> 200 nm，类似火星的大气）中的样品接收的总紫外线（200-400） nm）的能量密度分别约为4.58 x 10（2）kJ / m（2）和4.92 x 10（2）kJ / m（2），以及0.5 Gy的宇宙电离辐射。由于太空任务的持续时间（从发射到样品返回实验室），对2.5岁的样品进行了飞行后分析。通过聚合酶链反应停止试验和共聚焦激光扫描显微镜评估补液后细胞分裂以及对基因组和光合装置的损伤，确定存活细胞的存在。生物膜的恢复性优于浮游生物，不仅在太空和类似火星的条件下暴露于紫外线辐射下，而且在黑暗的环境下以及低地球条件和实验室控制条件下暴露时，其累积的损伤也较小。这表明，尽管由顶层细胞层提供的屏蔽足以使多层浮游生物样品在一定程度上存活，但生物膜的存活率却提高了，这是由于存在大量的细胞外聚合物质以及干燥后获得的其他特征。

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  • 来源
    《Astrobiology》 |2019年第8期|1008-1017|共10页
  • 作者

    Billi Daniela; Staibano Clelia; Verseux Cyprien; Fagliarone Claudia; Mosca Claudia; Baque Mickael; Rabbow Elke; Rettberg Petra;

  • 作者单位

    Univ Roma Tor Vergata, Dept Biol, Via Ric Sci, I-00133 Rome, Italy;

    Univ Roma Tor Vergata, Dept Biol, Via Ric Sci, I-00133 Rome, Italy;

    Univ Roma Tor Vergata, Dept Biol, Via Ric Sci, I-00133 Rome, Italy;

    Univ Roma Tor Vergata, Dept Biol, Via Ric Sci, I-00133 Rome, Italy;

    Univ Roma Tor Vergata, Dept Biol, Via Ric Sci, I-00133 Rome, Italy;

    German Aerosp Ctr DLR, Inst Planetary Res Management & Infrastruct, Astrobiol Labs, Res Grp, Berlin, Germany;

    German Aerosp Ctr DLR, Inst Aerosp Med, Cologne, Germany;

    German Aerosp Ctr DLR, Inst Aerosp Med, Cologne, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Cyanobacteria; Mars-like; Space; Biofilms; Planktonic; EXPOSE;

    机译：蓝藻;火星样;空间;生物膜;浮游;露出;

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