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Application of the mars organic analyzer to nucleobase and amine biomarker detection

机译：火星有机分析仪在核碱基和胺生物标志物检测中的应用

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摘要

The Mars Organic Analyzer (MOA), a portable microfabricated capillary electrophoresis instrument being developed for planetary exploration, is used to analyze a wide variety of fluorescamine-labeled amine-containing biomarker compounds, including amino acids, mono-and diaminoalkanes, amino sugars, nucleobases, and nucleobase degradation products. The nucleobases cytosine and adenine, which contain an exocyclic primary amine, were effectively labeled, separated, and detected at concentrations < 500 nM. To test the general applicability of the MOA for biomarker detection, amino acids and mono- and diamines were extracted from bacterial cells using both hydrolysis and sublimation followed by analysis. The extrapolated limit of detection provided by the valine biomarker was similar to 4 x 10(3) cells per sample. Products of an NH4CN polymerization that simulate a prebiotic synthesis were also successfully isolated via sublimation and analyzed. Adenine and alanine/serine were detected with no additional sample cleanup at 120 +/- 13 mu M and 4.1 +/- mu M, respectively, corresponding to a reaction yield of 0.04% and 0.0003%, respectively. This study demonstrates that the MOA provides sensitive detection and analysis of low levels of a wide variety of amine-containing organic compounds from both biological and abiotic sources.
机译：火星有机分析仪（MOA）是为行星探测而开发的便携式微型毛细管电泳仪，用于分析多种荧光胺标记的含胺生物标记化合物，包括氨基酸，单和二氨基烷烃，氨基糖，核碱基和核碱基降解产物。含有胞外伯胺的核苷胞嘧啶和腺嘌呤经过有效标记，分离和检测，浓度小于500 nM。为了测试MOA在生物标志物检测中的一般适用性，使用水解和升华方法从细菌细胞中提取氨基酸以及一元和二元胺，然后进行分析。缬氨酸生物标志物提供的外推检测限类似于每个样品4 x 10（3）个细胞。通过升华并分析成功地模拟了模拟益生元合成的NH4CN聚合产物。检测到腺嘌呤和丙氨酸/丝氨酸后，分别在120 +/- 13μM和4.1 +/-μM的样品上没有额外的样品净化，分别对应于0.04％和0.0003％的反应产率。这项研究表明，MOA可对来自生物和非生物来源的多种含胺有机化合物的低含量进行灵敏的检测和分析。

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