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Methane production by methanogens following an aerobic washing procedure: Simplifying methods for manipulation

机译：需氧洗涤程序后产甲烷菌产生的甲烷：简化操作方法

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The recent discovery of methane in the martian atmosphere is arguably one of the most important discoveries in the field of astrobiology. One possible source of this methane could be a microorganism analogous to those on Earth in the domain Archaea known as methanogens. Methanogens are described as obligately anaerobic, and methods developed to work with methanogens typically include anaerobic media and buffers, gassing manifolds, and possibly anaerobic chambers. To determine if the time, effort, and supplies required to maintain anaerobic conditions are necessary to maintain viability, we compared anaerobically washed cells with cells that were washed in the presence of atmospheric oxygen. Anaerobic tubes were opened, and cultures were poured into plastic centrifuge tubes, centrifuged, and suspended in fresh buffer, all in the presence of atmospheric oxygen. Washed cells from both aerobic and anaerobic procedures were inoculated into methanogenic growth media under anaerobic conditions and incubated at temperatures conducive to growth for each methanogenic strain tested. Methane production was measured at time intervals using a gas chromatograph. In three strains, significant differences were not seen between aerobically and anaerobically washed cells. In one strain, there was significantly less methane production observed following aerobic washing at some time points; however, substantial methane production occurred following both procedures. Thus, it appears that aerobic manipulations for relatively short periods of time with at least a few species of methanogens may not lead to loss of viability. With the discovery of methane in the martian atmosphere, it is likely that there will be an increase in astrobiology-related methanogen research. The research reported here should simplify the methodology.
机译：火星大气中甲烷的最新发现可以说是占星生物学领域最重要的发现之一。这种甲烷的一种可能来源可能是一种类似于古生菌领域中地球上被称为产甲烷菌的微生物。产甲烷菌被描述为专性厌氧，而开发用于产甲烷菌的方法通常包括厌氧培养基和缓冲液，排气歧管以及可能的厌氧室。为了确定维持厌氧条件所需的时间，精力和耗材对于维持生存能力是否必要，我们将厌氧洗涤过的细胞与在大气氧存在下洗涤过的细胞进行了比较。打开厌氧管，将培养物倒入塑料离心管中，离心，并悬浮在新鲜缓冲液中，全部在大气氧存在下。将来自有氧和厌氧程序的洗涤细胞在厌氧条件下接种到产甲烷的生长培养基中，并在每个测试产甲烷菌株的有利于生长的温度下孵育。使用气相色谱仪以时间间隔测量甲烷的产生。在三种菌株中，需氧和需氧洗涤细胞之间没有发现显着差异。在一种菌株中，在某些时间点进行好氧洗涤后观察到的甲烷生成量明显减少。然而，两种方法都产生了大量的甲烷。因此，似乎在至少较短的时间内用至少几种产甲烷菌进行有氧操作可能不会导致生存力下降。随着火星大气层中甲烷的发现，与天文生物学相关的产甲烷原的研究可能会增加。此处报道的研究应简化方法。

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