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首页> 外文期刊>Biocatalysis and Biotransformation >Biotechnological production of poly(3-hydroxybutyrate) with Wautersia eutropha by application of green grass juice and silage juice as additional complex substrates
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Biotechnological production of poly(3-hydroxybutyrate) with Wautersia eutropha by application of green grass juice and silage juice as additional complex substrates

机译：通过使用绿草汁和青贮汁汁作为额外的复杂底物，以富营养的Wautersia富营养化生产聚3-羟基丁酸酯

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摘要

Alternative inexpensive complex nitrogen- and phosphate sources from agriculture, green grass juice (GGJ) and silage juice (SJ), were added to cultivation medium in order to investigate their impact on growth of the well-known polyhydroxyalk-anoate (PHA) accumulating strain Wautersia eutropha. The influence of these additives was directly compared with cultivations on defined minimal mineral medium (M) as well as on the same medium supplemented with more expensive complex additives: corn steep liquor (CSL) and casamino acids (CA). It turned out that the supplementation with most complex additives results in shortening of lag-phases of bacterial growth and in higher end-concentrations of residual biomass compared with M-medium. Finally, higher volumetric productivities for poly(3-hydroxybutyrate) (3-PHB) were achieved. The effect of the inexpensive additive SJ on volumetric productivity was similar to the result for the expensive CA (0.653 vs. 0.619 g L~(-1) h~(-1)). The same was found for the biomass concentration (7.00 vs. 7.44 g L~(-1) respectively). Together with an economic appraisal presented in this study, the results suggest it is possible to make the sustainable process of microbial PHA-production more economically feasible. A survey of the thermal characteristics and molecular mass properties of the isolated polymers completes this work.
机译：为了研究它们对著名的聚羟基链烷酸（PHA）积累菌株生长的影响，向农业培养基中添加了来自农业，绿草汁（GGJ）和青贮汁（SJ）的其他廉价的复杂氮和磷酸盐源。富营养度。将这些添加剂的影响与确定的基本矿物质培养基（M）以及补充了较昂贵的复杂添加剂的相同培养基（玉米浆（CSL）和酪蛋白氨基酸（CA））上的种植直接进行比较。事实证明，与M培养基相比，添加最复杂的添加剂可缩短细菌生长的滞后期，并提高残留生物量的最终浓度。最后，获得了更高的聚（3-羟基丁酸酯）（3-PHB）的体积生产率。廉价的添加剂SJ对体积生产率的影响类似于昂贵的CA的结果（0.653对0.619 g L〜（-1）h〜（-1））。发现生物质浓度相同（分别为7.00对7.44 g L〜（-1））。连同本研究中提出的经济评估，结果表明有可能使微生物PHA生产的可持续过程在经济上更加可行。对分离出的聚合物的热特性和分子量性质的调查完成了这项工作。

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