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Cloning and Identification of MicroRNAs in Earthworm (Eisenia fetida)

机译：worm（Eisenia fetida）中微小RNA的克隆与鉴定

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MicroRNAs (miRNAs) (noncoding RNAs of 20–25 nucleotides) play important roles in the post-transcriptional regulation of gene expression in various eukaryotes and prokaryotes. Piwi-interacting RNAs function by combining with PIWI proteins to regulate protein synthesis and to stabilize mRNA, the chromatin framework, and genome structure. This study investigates the role of miRNAs in regeneration. A scrDNA library was constructed, and 17 noncoding RNAs from Eisenia fetida (an optimal model for the study of earthworm regeneration) were cloned and characterized. In addition, reverse transcription polymerase chain reaction was performed to analyze the expression of four small RNAs during different developmental stages. The expression levels of these RNAs in regenerating tissue were higher than in normal tissue, and the expression patterns of these small RNAs were unique during development.
机译：MicroRNA（miRNA）（20-25个核苷酸的非编码RNA）在转录后调控各种真核生物和原核生物的基因表达中起重要作用。与Piwi相互作用的RNA通过与PIWI蛋白结合来调节蛋白合成并稳定mRNA，染色质框架和基因组结构。这项研究调查了miRNA在再生中的作用。构建了一个scrDNA文库，并克隆和鉴定了17个来自Eisenia fetida的非编码RNA（研究worm再生的最佳模型）。另外，进行了逆转录聚合酶链反应以分析四个小RNA在不同发育阶段的表达。这些RNA在再生组织中的表达水平高于正常组织，并且这些小RNA的表达模式在发育过程中是独特的。

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