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首页> 外文期刊>Biochemical Genetics >Two Different Size Classes of 5S rDNA Units Coexisting in the Same Tandem Array in the Razor Clam Ensismacha: Is This Region Suitable for Phylogeographic Studies?
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Two Different Size Classes of 5S rDNA Units Coexisting in the Same Tandem Array in the Razor Clam Ensismacha: Is This Region Suitable for Phylogeographic Studies?

机译：在剃刀蛤Ensismacha中以相同的串联阵列共存的两种不同大小的5S rDNA单元类别：该区域是否适合植物志研究？

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摘要

For a study of 5S ribosomal genes (rDNA) in the razor clam Ensis macha, the 5S rDNA region was amplified and sequenced. Two variants, so-called type I or short repeat (~430 bp) and type II or long repeat (~735 bp), appeared to be the main components of the 5S rDNA of this species. Their spacers differed markedly, both in length and nucleotide composition. The organization of the two variants was investigated by amplifying the genomic DNA with primers based on the sequence of the type I and type II spacers. PCR amplification products with primers EMLbF and EMSbR showed that the long and short repeats are associated within the same tandem array, suggesting an intermixed arrangement of both spacers. Nevertheless, amplifications carried out with inverse primers EMSinvF/R and EMLinvF/R revealed that some short and long repeats are contiguous in the same tandem array. This is the first report of the coexistence of two variable spacers in the same tandem array in bivalve mollusks. Keywords 5S rDNA - Nontranscribed spacer variations - Cluster organization - Razor clam - Ensis macha Juan Fernández-Tajes and Josefina Méndez have contributed equally to this work.
机译：为了研究剃刀蛤Ensis macha中的5S核糖体基因（rDNA），对5S rDNA区域进行了扩增和测序。两种变体，即I型或短重复（〜430 bp）和II型或长重复（〜735 bp），似乎是该物种5S rDNA的主要成分。它们的间隔区在长度和核苷酸组成上都明显不同。通过基于I型和II型间隔子的序列的引物扩增基因组DNA，研究了两个变体的组织。具有引物EMLbF和EMSbR的PCR扩增产物表明，长重复和短重复在同一串联阵列内相关，表明两个间隔子的混合排列。然而，使用反向引物EMSinvF / R和EMLinvF / R进行的扩增显示，在同一串联阵列中一些短重复和长重复是连续的。这是双壳软体动物在同一串联阵列中两个可变间隔子共存的首次报道。关键字5S rDNA-非转录间隔区变异-簇组织-剃刀蛤-恩西斯·玛查JuanFernández-Tajes和JosefinaMéndez对这项工作做出了同样的贡献。

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