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首页> 外文期刊>Biochemical Genetics >Cloning and Characterization of the SSB-1 and SSB-4 Genes Expressed in Zebrafish Gonads
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Cloning and Characterization of the SSB-1 and SSB-4 Genes Expressed in Zebrafish Gonads

机译：斑马鱼性腺中表达的SSB-1和SSB-4基因的克隆与鉴定

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The protein of the gustavus (gus) gene has a typical SOCS box domain and repeats in the splA and RyR (SPRY) domains. GUS can interact with Vasa and is necessary for the specification of germ cells. We cloned two zebrafish genes, SSB-1 and SSB-4 (SPRY domain SOCS box proteins). Phylogenetic analysis shows that zebrafish SSB-1 and SSB-4 are clustered into clades of SSB-1-like and SSB-4-like genes from other species. RT-PCR analysis of tissues revealed that zebrafish SSB-1 and -4 are expressed in the ovary and testis. We investigated the spatial expression patterns of zebrafish SSB-1 and -4 in embryos from the two-cell stage to 72 h postfertilization (hpf) using whole-mount in situ hybridization. SSB-1 and -4 transcripts were present in all blastomeres during the early embryonic stages, but the genes differ in their expression pattern. SSB-4 mRNA was located in the region of the primordial germ cells in 24 and 72 hpf embryos, but SSB-1 mRNA was not detected at these stages. We hypothesize that SSB-4 plays a role in the early development of germ cells. Keywords Zebrafish - SSB-1 - SSB-4 - Gus - Primordial germ cells
机译：gustavus（gus）基因的蛋白质具有典型的SOCS框结构域，并在splA和RyR（SPRY）结构域中重复。 GUS可以与Vasa相互作用，并且对于规范生殖细胞是必需的。我们克隆了两个斑马鱼基因，SSB-1和SSB-4（SPRY域SOCS框蛋白）。系统发育分析表明，斑马鱼SSB-1和SSB-4聚集成了其他物种的SSB-1类和SSB-4类基因。组织的RT-PCR分析显示，斑马鱼SSB-1和-4在卵巢和睾丸中表达。我们调查了斑马鱼SSB-1和-4在胚胎从两细胞阶段到受精后72 h（hpf）使用全安装原位杂交的空间表达模式。在胚胎早期，所有卵裂球中都存在SSB-1和-4转录本，但是这些基因的表达方式不同。 SSB-4 mRNA位于24和72 hpf胚胎的原始生殖细胞区域，但在这些阶段未检测到SSB-1 mRNA。我们假设SSB-4在生殖细胞的早期发育中起作用。斑马鱼-SSB-1-SSB-4-Gus-原始生殖细胞

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