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首页> 外文期刊>Biochemical Genetics >Genetic Assessment of Traits and Genetic Relationship in Blackgram (Vigna mungo) Revealed by Isoenzymes
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Genetic Assessment of Traits and Genetic Relationship in Blackgram (Vigna mungo) Revealed by Isoenzymes

机译：同工酶揭示黑豆（Vigna mungo）性状的遗传评估和遗传关系

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摘要

Sixty blackgram accessions were evaluated and classified into different clusters to assess genetic diversity and traits using isoenzymes. Trait-specific expression was assessed, and isoenzyme bands were observed: a peroxidase band (Rm 0.60) associated with dwarfness and an esterase band (Rm 0.25) with tallness. Early maturing varieties were characterized by a specific esterase isoenzyme band of Rm 0.51. All yellow mosaic virus susceptible genotypes had two bands of esterase isoenzyme, Rm 0.42 and 0.70. Resistant genotypes showed three bands (0.32, 0.33, and 0.35) of alkaline phosphatase. Peroxidase isoenzyme was helpful to differentiate green-seeded from black-seeded varieties. Two bands (0.58 and 0.83) were observed in black-seeded accessions, and two different bands (0.74 and 0.76) were observed in green-seeded accessions. Clustering of germplasm and assessment of traits will facilitate the use of germplasm for the improvement of blackgram. Keywords Isoenzyme - Blackgram - Cluster analysis - Dendrogram - Peroxidase - Esterase - Alkaline Phosphatase
机译：评估了60个黑gram的种质，并分类为不同的簇，以利用同工酶评估遗传多样性和性状。特质表达的评估，并观察到同工酶带：与矮度相关的过氧化物酶带（Rm 0.60）和与矮度相关的酯酶带（Rm 0.25）。早熟品种的特征是Rm 0.51的特定酯酶同工酶带。所有黄色花叶病毒易感基因型都有两个酯酶同工酶带，Rm为0.42和0.70。抗性基因型显示了碱性磷酸酶的三个条带（0.32、0.33和0.35）。过氧化物酶同工酶有助于区分绿色种子和黑色种子。在黑种质中观察到两个条带（0.58和0.83），在绿种质中观察到两个不同的条带（0.74和0.76）。种质的聚类和性状的评估将促进种质的使用，以改善黑麦草。同工酶-Blackgram-聚类分析-树状图-过氧化物酶-酯酶-碱性磷酸酶

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