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首页> 外文期刊>Biochemical Genetics >Biological and Molecular Characterization of a Crucifer Tobamovirus Infecting Oilseed Rape
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Biological and Molecular Characterization of a Crucifer Tobamovirus Infecting Oilseed Rape

机译：十字花科烟草花叶病毒感染油菜的生物学和分子特征。

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In China, the tobamovirus that infects oilseed rape has been misdiagnosed as Tobacco mosaic virus (TMV) based on its morphological similarity and serological relatedness. Recently, a tobamovirus has been isolated from oilseed rape in China, which we named Youcai mosaic virus Br (YoMV-Br), according to its biological and molecular characteristics. It had strong infectivity to Cruciferae but less to Solanaceae, Leguminosae, and Cucurbitaceae, and its virion morphology was consistent with that of the tobamoviruses. At high concentrations, it serologically cross reacted with TMV antiserum. The 3′ terminal sequence (2,283 nucleotides) of YoMV-Br was determined, including the 3′ noncoding region, the CP and MP genes, and the C-terminal part of the replicase gene. Between the MP and CP genes, 77 nucleotides overlapped. Compared with homologous regions of 21 recognized species of Tobamovirus, YoMV-Br had a much higher identity to crucifer species than to other tobamoviruses. Phylogenetic analysis demonstrated that YoMV-Br was closely related to the YoMV cluster of tobamoviruses and distantly to TMV, so that they likely belong to different strains of the same species. Keywords Youcai mosaic virus (YoMV) - Serology - Sequence - Phylogenetic analysis
机译：在中国，基于油菜籽的形态相似性和血清学相似性，其感染油菜的烟草花叶病毒被误诊为烟草花叶病毒（TMV）。最近，从油菜中分离到一种烟草花叶病毒，根据其生物学和分子特征，我们将其命名为油菜花叶病毒Br（YoMV-Br）。它对十字花科具有较强的感染力，而对茄科，豆科和葫芦科的感染力较小，其病毒体形态与烟草花叶病毒一致。在高浓度下，它与TMV抗血清发生血清学交叉反应。确定了YoMV-Br的3'末端序列（2,283个核苷酸），包括3'非编码区，CP和MP基因以及复制酶基因的C末端部分。在MP和CP基因之间，有77个核苷酸重叠。与21种公认的Tobamovirus物种的同源区域相比，YoMV-Br与十字花科物种的同源性远高于与其他Tobamovirus的同源性。系统发育分析表明，YoMV-Br与烟草花叶病毒的YoMV簇密切相关，而与TMV则远缘，因此它们可能属于同一物种的不同菌株。优彩花叶病毒（YoMV）-血清学-序列-系统发育分析

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