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首页> 外文期刊>Biochemical Genetics >Polymorphism of the Porcine Leptin Gene Promoter and Analysis of Its Association with Gene Expression and Fatness Traits
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Polymorphism of the Porcine Leptin Gene Promoter and Analysis of Its Association with Gene Expression and Fatness Traits

机译：猪瘦素基因启动子的多态性及其与基因表达和脂肪性状的关联分析

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摘要

We describe for the first time a 245 bp fragment of the porcine leptin gene promoter in the proximity of the transcription start site. Altogether, 720 pigs were screened with the PCR-SSCP technique for polymorphism in this region. Four SNPs, segregating as two haplotypes, have been identified, one of them (C113G) in the putative consensus site for the AP-2 transcription factor. This polymorphism was evenly distributed in the Duroc breed (n=21) and was absent in the Polish Landrace (n=248) and Pietrain breed (n=12). In the Polish Large White (n=191) and synthetic line 990 (n=243), allele G occurred with a very low frequency. The investigation was performed to test if the C113G SNP affects leptin mRNA level in subcutaneous fat and leptin protein concentration in serum. Additionally, the effect of this polymorphism on fatness traits was statistically analyzed. Although there was a trend toward decreased expression in GG animals, the differences were not significant between genotypes. We also found no evidence for an association of the LEP promoter genotype with the analyzed fatness traits.
机译：我们第一次描述了转录起始位点附近的猪瘦素基因启动子的245 bp片段。用PCR-SSCP技术筛选了720头猪的多态性。已鉴定出四个SNP，它们被分离为两个单倍型，其中一个（C113G）位于AP-2转录因子的假定共有位点。这种多态性在杜洛克（Duroc）品种（n = 21）中均匀分布，而在波兰长白（n = 248）和皮特兰（Pietrain）品种（n = 12）中则不存在。在波兰大白（n = 191）和合成系990（n = 243）中，等位基因G的发生频率非常低。进行研究以测试C113G SNP是否影响皮下脂肪中瘦素mRNA水平和血清中瘦素蛋白浓度。另外，统计分析了这种多态性对肥胖性状的影响。尽管在GG动物中有表达减少的趋势，但基因型之间的差异并不明显。我们还没有发现证据表明LEP启动子基因型与所分析的脂肪性状相关。

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  • 来源
    《Biochemical Genetics》 |2007年第4期|245-253|共9页
  • 作者

    M. Stachowiak; M. Mackowski; Z. Madeja; M. Szydlowski; A. Buszka; P. Kaczmarek; B. Rubis; P. Mackowiak; K. W. Nowak; M. Switonski;

  • 作者单位

    Department of Genetics and Animal Breeding Agricultural University of Poznan Poznan Poland;

    Department of Genetics and Animal Breeding Agricultural University of Poznan Poznan Poland;

    Department of Genetics and Animal Breeding Agricultural University of Poznan Poznan Poland;

    Department of Genetics and Animal Breeding Agricultural University of Poznan Poznan Poland;

    Department of Genetics and Animal Breeding Agricultural University of Poznan Poznan Poland;

    Department of Animal Physiology and Biochemistry Agricultural University of Poznan Poznan Poland;

    Department of Clinical Chemistry University of Medical Sciences Poznan Poland;

    Department of Animal Physiology and Biochemistry Agricultural University of Poznan Poznan Poland;

    Department of Animal Physiology and Biochemistry Agricultural University of Poznan Poznan Poland;

    Department of Genetics and Animal Breeding Agricultural University of Poznan Poznan Poland;

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  • 正文语种 eng
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  • 关键词

    leptin promoter; pig; fat; single nucleotide polymorphism (SNP); quantitative trait locus (QTL);

    机译：瘦素启动子;猪;脂肪;单核苷酸多态性（SNP）;数量性状位点（QTL）;

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