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首页> 外文期刊>Biochemical Genetics >Effect of New SNP Within Bovine Prolactin Gene Enhancer Region on Expression in the Pituitary Gland
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Effect of New SNP Within Bovine Prolactin Gene Enhancer Region on Expression in the Pituitary Gland

机译：牛催乳激素基因增强子区内新的SNP对垂体表达的影响

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摘要

A new single nucleotide polymorphism was revealed using PCR–SSCP and sequencing methods within the bovine prolactin distal promoter region described as a functional enhancer. The A→G transition at position −1043 abolishes the recognition site for Hsp92II restriction endonuclease, allowing for PCR–RFLP genotyping. The application of real-time PCR revealed that the prolactin gene expression level in the pituitary was higher in cattle with the AA genotype than in those with the GG genotype. EMSA analysis, however, showed increased nuclear protein binding to the sequence variant with G, suggesting a possible inhibition event, in which the transcription factors Pit1, Oct1, and YY1 could be involved.
机译：使用PCR-SSCP和测序方法揭示了一种新的单核苷酸多态性，该方法在被称为功能增强子的牛催乳激素远端启动子区域内。在位置−1043处的A→G跃迁取消了Hsp92II限制性核酸内切酶的识别位点，可进行PCR-RFLP基因分型。实时PCR的应用表明，AA基因型牛的垂体催乳素基因表达水平高于GG基因型牛。然而，EMSA分析显示核蛋白与G序列变异的结合增加，表明可能存在抑制事件，其中可能涉及转录因子Pit1，Oct1和YY1。

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  • 来源
    《Biochemical Genetics》 |2007年第10期|743-754|共12页
  • 作者

    P. Brym; T. Malewski; R. Starzyński; K. Flisikowski; E. Wójcik; A. Ruść; L. Zwierzchowski; S. Kamiński;

  • 作者单位

    Department of Animal Genetics University of Warmia and Mazury Olsztyn Poland;

    Institute of Genetics and Animal Breeding Polish Academy of Sciences Jastrzębiec Wólka Kosowska Poland;

    Institute of Genetics and Animal Breeding Polish Academy of Sciences Jastrzębiec Wólka Kosowska Poland;

    Institute of Genetics and Animal Breeding Polish Academy of Sciences Jastrzębiec Wólka Kosowska Poland;

    Department of Animal Genetics University of Warmia and Mazury Olsztyn Poland;

    Department of Animal Genetics University of Warmia and Mazury Olsztyn Poland;

    Institute of Genetics and Animal Breeding Polish Academy of Sciences Jastrzębiec Wólka Kosowska Poland;

    Department of Animal Genetics University of Warmia and Mazury Olsztyn Poland;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Bovine prolactin; SNP; Promoter; Gene expression;

    机译：牛催乳素;SNP;启动子;基因表达;

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