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首页> 外文期刊>Biochemical Genetics >Genetic Heterogeneity of the Blue Swimming Crab (Portunus pelagicus) in Thailand Determined by AFLP Analysis
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Genetic Heterogeneity of the Blue Swimming Crab (Portunus pelagicus) in Thailand Determined by AFLP Analysis

机译:AFLP分析确定泰国蓝色游泳蟹(Portunus pelagicus)的遗传异质性

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摘要

Genetic diversity and population differentiation of the blue swimming crab (Portunus pelagicus) in Thailand, originating from Ranong and Krabi located in the Andaman Sea (west) and Chanthaburi, Prachuap Khiri Khan, and Suratthani located in the Gulf of Thailand (east), were examined by AFLP analysis. High genetic diversity of P. pelagicus in Thai waters was found (N = 72). The four primer combinations generated 227 AFLP fragments, and the percentage of polymorphic bands in each geographic sample was 66.19–94.38%. The mean genetic distance between pairs of samples was 0.1151–0.2440. Geographic heterogeneity analyses using the exact test and F ST-based statistics between all pairwise comparisons were statistically significant (P < 0.01), indicating a fine-scale level of intraspecific population differentiation of Thai P. pelagicus. The estimated number of migrants per generation (N e m) was 0.26–0.76, suggesting restricted gene flow levels of P. pelagicus in Thai waters.
机译:来自泰国安达曼海(西部)的拉廊和甲米以及位于泰国湾(东部)的尖竹汶府,班武里府和素叻府的泰国蓝色游泳蟹(Portunus pelagicus)的遗传多样性和种群分化通过AFLP分析进行了检查。在泰国水域发现了P. pelagicus的高度遗传多样性(N = 72)。四种引物组合产生了227个AFLP片段,每个地理样本中的多态性条带百分比为66.19–94.38%。两对样本之间的平均遗传距离为0.1151–0.2440。在所有成对比较之间使用精确检验和基于F ST 的统计数据进行的地理异质性分析具有统计学显着性(P <0.01),表明泰国P. pelagicus种内种群分化的精细水平。每代移民的估计数量(N e m)为0.26-0.76,这表明泰国水域中P. pelagicus的基因流量水平受到限制。

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