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首页> 外文期刊>Biochemical Genetics >Isoenzyme Variation in Melipona rufiventris (Hymenoptera: Apidae, Meliponina) in Minas Gerais State, Brazil
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Isoenzyme Variation in Melipona rufiventris (Hymenoptera: Apidae, Meliponina) in Minas Gerais State, Brazil

机译：巴西米纳斯吉拉斯州美黑锦蛇（膜翅目：Apidae，Meliponina）的同工酶变异

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摘要

The stingless bee Melipona rufiventris is an important pollinator in several Brazilian ecosystems. Originally widely distributed in Minas Gerais (MG) state, this species is becoming very rare. Therefore this species was included in the endangered species list of MG. We used isoenzyme data for a better understanding of the genetic structure of several M. rufiventris colonies. Samples of 35 colonies were collected from 12 localities and evaluated by nine enzymatic systems, which yielded 17 loci. M. rufiventris genetic variation was found to be low, typical of an endangered species. The proportion of polymorphic loci was 5.88% in both ecosystems. Only Est-4 was polymorphic in colonies from the Forest and Mdh-1 in colonies from the Cerrado. The expected heterozygosity ranged from 0.0068 in the Cerrado to 0.0078 in the Forest. Despite this, enzyme electrophoretic analyses provided a good idea of the diversity between samples from Cerrado and Forest which reinforce the existence of two different “forms” of M. rufiventris in MG, one present in the Cerrado and the other in Forest. This information is of great importance for the conservation of, M. rufiventris in MG.
机译：无刺蜂Melipona rufiventris是巴西几个生态系统中的重要传粉者。最初在米纳斯吉拉斯州（MG）广泛分布，该物种变得非常稀有。因此，该物种被列入MG的濒危物种清单。我们使用同工酶数据更好地了解了几个rufiventris菌落的遗传结构。从12个地点收集了35个菌落的样品，并通过9个酶系统进行了评估，产生了17个基因座。发现rufiventris的遗传变异低，典型的濒危物种。在两个生态系统中，多态位点的比例为5.88％。在森林的殖民地中只有Est-4是多态性的，在塞拉多的殖民地中只有Mdh-1。预期的杂合度从Cerrado的0.0068到Forest的0.0078。尽管如此，酶电泳分析还是对Cerrado和Forest样品之间多样性的一个好主意，这加强了MG中rufiventris的两种不同“形式”的存在，一种存在于Cerrado中，另一种存在于Forest中。此信息对于保护MG中的rufiventris至关重要。

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