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首页> 外文期刊>Biochemical Genetics >Induction of Suppressed ADH Activity in Drosophila pachea Exposed to Different Alcohols
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Induction of Suppressed ADH Activity in Drosophila pachea Exposed to Different Alcohols

机译：暴露于不同酒精的果蝇中抑制ADH活性的诱导

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摘要

Laboratory-reared males of the cactophilic Drosophila pachea exhibit a spontaneous and sex-specific suppression of alcohol dehydrogenase (ADH) activity within 4 days after eclosion. A lack of ADH activity also is usually seen in wild-caught males, although relatively high activity is always seen in female flies. In the present study we examined the effectiveness of different alcohols and related compounds, including several found naturally in necroses of the host cactus, to induce suppressed ADH activity in wild males of D. pachea and to serve as enzyme substrates. The primary alcohols (methanol, ethanol, 1-propanol, 1-butanol, and 1-pentanol), and the secondary alcohols (2-propanol and 2-butanol), each induced activity after 24 h exposure, although to different degrees. 1,2-Propanediol was usually effective as an inducer, but 2,3-butanediol usually was ineffective. Little or no induction was seen with 1-octanol, 2-pentanol, 3-methyl-1-butanol, 3-hydroxy-2-butanone, or acetaldehyde. Although the compounds tested varied in their ability to function as ADH substrates, methanol was the only alcohol that showed no activity staining. Ethanol induction of ADH activity was apparent after 3–6 h exposure and induced activity decreased dramatically within 1 week of flies being placed in an alcohol-free environment. Ethanol exposure did not induce ADH in adult female D. pachea, or in adult males and females of D. acutilabella in which control males show reduced ADH activity compared to females. The implications of the loss of ADH activity in adult males of D. pachea, as they relate to feeding ecology and fitness, are discussed.
机译：实验室饲养的雄性果蝇果蝇的雄性在羽化后4天内表现出自发性和性别特异性抑制酒精脱氢酶（ADH）活性。在野外捕获的雄性动物中通常也缺乏ADH活性，尽管在雌性蝇中总是发现相对较高的活性。在本研究中，我们研究了不同的醇类和相关化合物（包括在宿主仙人掌坏死中天然发现的几种化合物）在诱导的D. pachea雄性雄性中抑制ADH活性并用作酶底物的有效性。伯醇（甲醇，乙醇，1-丙醇，1-丁醇和1-戊醇）和仲醇（2-丙醇和2-丁醇）在暴露24小时后均会诱导活性，尽管程度不同。 1,2-丙二醇通常可有效用作诱导剂，但2,3-丁二醇通常无效。用1-辛醇，2-戊醇，3-甲基-1-丁醇，3-羟基-2-丁酮或乙醛几乎没有诱导。尽管所测试的化合物充当ADH底物的能力各不相同，但甲醇是唯一没有活性染色的醇。乙醇在暴露3至6小时后对ADH活性的诱导作用明显，诱导的活性在将果蝇置于无酒精的环境中1周内急剧下降。乙醇暴露在成年雌性D. pachea或成年雄性D. acutilabella的雌性中均不诱导ADH，其中对照雄性与雌性相比，ADH活性降低。讨论了D. pachea成年雄性中ADH活性丧失的意义，因为它们与摄食的生态和健康有关。

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