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首页> 外文期刊>Biochemical Genetics >Prophenol Oxidase A3 in Drosophila melanogaster: Activation and the PCR-Based cDNA Sequence
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Prophenol Oxidase A3 in Drosophila melanogaster: Activation and the PCR-Based cDNA Sequence

机译：果蝇中的前酚氧化酶A3 ：激活和基于PCR的cDNA序列

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摘要

Phenol oxidase exists in Drosophila hemolymph as a prophenol oxidase, A1 and A3, that is activated in vivo with a native activating system, AMM-1, by limited proteolysis with time. The polypeptide in purified prophenol oxidase A3 has a molecular weight of approximately 77,000 Da. A PCR-based cDNA sequence coding A3 has 2501 bp encoding an open reading frame of 682 amino acid residues. The potential copper-binding sites, from Trp-196 to Tyr-245, and from Asn-366 to Phe-421, are highly homologous to the corresponding sites in other invertebrates. The availability of prophenol oxidase cDNA should be useful in revealing the biochemical differences between A1 and A3 isoforms in Drosophila melanogaster that are refractory or unable to activate prophenol oxidase.
机译：果蝇血淋巴中的苯酚氧化酶以一种前酚氧化酶A1和A3的形式存在，并通过有限时间的蛋白水解作用在体内被天然激活系统AMM-1激活。纯化的前酚氧化酶A3 中的多肽的分子量约为77,000 Da。基于PCR的cDNA编码A3 的序列长为2501 bp，编码682个氨基酸残基的开放阅读框。从Trp-196到Tyr-245，从Asn-366到Phe-421的潜在铜结合位点与其他无脊椎动物中的相应位点高度同源。前酚氧化酶cDNA的可用性应有助于揭示果蝇中难治或无法激活前酚氧化酶的A1亚型和A3亚型之间的生化差异。

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