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首页> 外文期刊>Biochemical Genetics >Genetic Variability in Hoplias malabaricus (Osteichthyes: Erythrinidae) in Fluvial and Lacustrine Environments in the Upper Paraná River Floodplain (Paraná State, Brazil)
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Genetic Variability in Hoplias malabaricus (Osteichthyes: Erythrinidae) in Fluvial and Lacustrine Environments in the Upper Paraná River Floodplain (Paraná State, Brazil)

机译：巴拉那河上游平原（巴西帕拉纳州）河道和湖泊环境中的Hoplias malabaricus（Osteichthyes：Erythrinidae）的遗传变异性

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摘要

Genetic variability in Hoplias malabaricus, from two localities in the upper Paraná River floodplain, was investigated by starch and polyacrylamide gel electrophoresis. A total of 52 specimens were analyzed for 14 enzymatic systems. Twenty-three gene loci of 13 enzymatic systems (AAT, ACP, ADH, GDH, G6PDH, GPI, IDH, LDH, MDH, MEP, PGM, PER, and SOD) were analyzed by starch gel electrophoresis (Penetrose-30). The EST system was analyzed by polyacrylamide gel electrophoresis, and one polymorphic locus was found (EST-1). Twenty-four loci were detected. The proportion of polymorphic loci was 37.5% in the lagoon and 33.3% in the river. Significant differences in allele frequencies of five loci were found between specimens from the two environments. Expected mean heterozygosity (H e = 0.14) is the same in the river and lagoon, however, Nei's genetic distance (D) between the population of the two locations was 0.049.
机译：通过淀粉和聚丙烯酰胺凝胶电泳研究了巴拉那河上游平原两处的Hoplias malabaricus的遗传变异性。总共分析了52个样本的14种酶系统。通过淀粉凝胶电泳（Penetrose-30）分析了13个酶系统的23个基因座（AAT，ACP，ADH，GDH，G6PDH，GPI，IDH，LDH，MDH，MEP，PGM，PER和SOD）。通过聚丙烯酰胺凝胶电泳分析EST系统，发现一个多态性基因座（EST-1）。检测到二十四个基因座。泻湖中多态性位点的比例为37.5％，河流中为33.3％。在两个环境的标本之间发现五个基因座的等位基因频率有显着差异。在河流和泻湖中，期望的平均杂合度（H e = 0.14）是相同的，但是，Nei在两个地点的种群之间的遗传距离（D）为0.049。

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