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首页> 外文期刊>Biochemical Genetics >Comparative Analysis of Genetic Diversity Among Indian Populations of Scirpophaga incertulas by ISSR-PCR and RAPD-PCR
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Comparative Analysis of Genetic Diversity Among Indian Populations of Scirpophaga incertulas by ISSR-PCR and RAPD-PCR

机译：用ISSR-PCR和RAPD-PCR比较印度小Sc皮种群遗传多样性

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摘要

Genetic variation between 28 Indian populations of the rice pest, Scirpophaga incertulas was evaluated using inter-simple sequence repeats (ISSR)-PCR assay. Nine SSR primers gave rise to 79 amplification products of which 67 were polymorphic. A dendrogram constructed from this data indicates that there is no geographical bias to the clustering and that gene flow between populations appears to be relatively unrestricted, substantiating our earlier conclusion based on the RAPD (random amplified polymorphic DNA) data. The dendrograms obtained using each of these marker systems were poorly correlated with each other as determined by Mantel's test for matrix correlation. Estimates of expected heterozygosity and marker index for each of these marker systems suggests that both these marker systems are equally efficient in determining polymorphisms. Matrix correlation analyses suggest that reliable estimates of genetic variation among the S. incertulas pest populations can be obtained by using RAPDs alone or in combination with ISSRs, but ISSRs alone cannot be used for this purpose.
机译：使用简单序列间重复（ISSR）-PCR分析法评估了印度28种水稻害虫稻草（Scirpophaga incertulas）之间的遗传变异。九个SSR引物产生了79个扩增产物，其中67个是多态性的。根据该数据构建的树状图表明，聚类没有地理偏向，种群之间的基因流似乎相对不受限制，这证实了我们先前基于RAPD（随机扩增的多态性DNA）数据得出的结论。使用Mantel的矩阵相关性测试确定，使用这些标记系统中的每一种获得的树状图之间的相关性很差。对这些标记系统中的每一个的预期杂合性和标记指数的估计表明，这两种标记系统在确定多态性方面同样有效。矩阵相关分析表明，可以单独使用RAPD或与ISSR结合使用，来获得不安全链球菌有害生物种群之间遗传变异的可靠估计值，但不能将ISSR单独用于此目的。

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