Using Ethidium To Probe Nonequilibrium States of DNA Condensed for Gene Delivery

Kristina Fant Bengt Norden and Per Lincoln

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Using Ethidium To Probe Nonequilibrium States of DNA Condensed for Gene Delivery

机译：使用Ethidium探测冷凝的非平衡态DNA的基因传递。

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Here we explore the use of ethidium to determinenrelative affinities of different gene delivery vectors for DNAnand describe an improved method for studying the inter-naction. Specifically, we investigate the binding of poly-n(amidoamine) dendrimers and show that the DNA-ndendrimer-ethidium system is far from thermodynamicnequilibrium. Moreover, dendrimer surface modificationnthrough PEGylation appears to make the interaction withnDNA more reversible, which is favorable fromthe perspec-ntive of vector unpacking. Probing the nonequilibrium statenof DNA during condensation processes is thus importantnfor developing novel vectors, and further, it could also benuseful in the study of chromatin folding.

机译：在这里，我们探索了使用乙啶来确定DNAn的不同基因传递载体的相对亲和力，并描述了一种研究相互作用的改进方法。具体来说，我们调查了聚正（胺基胺）树状聚合物的结合，并表明DNA-ndendrimer-乙锭体系远非热力学平衡。此外，通过聚乙二醇化的树枝状聚合物表面修饰似乎使与DNA的相互作用更可逆，这从载体解体的角度来看是有利的。因此，在缩合过程中探查DNA的非平衡状态对于开发新型载体很重要，而且，它对于染色质折叠的研究也可能是有益的。

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《Biochemistry》 |2011年第7期|p.1125-1127|共3页
作者
Kristina Fant Bengt Norden and Per Lincoln;
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Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96 G€ oteborg, Sweden;

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1. Using Ethidium To Probe Nonequilibrium States of DNA Condensed for Gene Delivery [J] . Fant K, Norden B, Lincoln P Biochemistry . 2011,第7期

机译：使用Ethidium探测浓缩的非平衡状态DNA的基因传递。
2. Delivery of Condensed DNA by Liposomal Non-viral Gene Delivery System into Nucleus of Dendritic Cells. [J] . Nakamura T, Moriguchi R, Kogure K, Biological & pharmaceutical bulletin . 2006,第6期

机译：通过脂质体非病毒基因递送系统将浓缩的DNA递送到树突状细胞核中。
3. Bone regeneration in a rat cranial defect with delivery of PEI-condensed plasmid DNA encoding for bone morphogenetic protein-4 (BMP-4). [J] . Huang YC, Simmons C, Kaigler D, Gene therapy . 2005,第5期

机译：大鼠颅骨缺损中的骨再生，并带有编码骨形态发生蛋白4（BMP-4）的PEI浓缩质粒DNA。
4. Delivery of PEI-condensed DNA via a porous polymer scaffold for tissue engineering applications [C] . Huang, Y., Mooney, . 2002

机译：通过多孔聚合物支架递送PEI缩合的DNA，用于组织工程应用
5. Structure-function investigations of DNA condensing agents with application to gene delivery. [D] . Evans, Heather Marie. 2005

机译：DNA缩合剂的结构功能研究及其在基因传递中的应用。
6. Nanoscopic structure of DNA condensed for gene delivery. [O] . D D Dunlap, A Maggi, M R Soria, 1997

机译：浓缩的DNA的纳米结构用于基因传递。
7. DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety [O] . Wagner Ernst, Cotten Matt, Mechtler Karl, 1991

机译：DNa结合转铁蛋白结合物作为功能基因递送剂：通过将聚赖氨酸或乙锭同型二聚体与转铁蛋白碳水化合物部分连接而合成
8. Hybridization of DNA Probes with Whole-Community Genome for Detection of Genes That Encode Microbial Responses to Pollutants: 'mer' Genes and Hg (2+) Resistance [R] . Barkay, T., Liebert, C., Gillman, M. 1989

机译：DNa探针与全社区基因组的杂交检测编码微生物对污染物的反应的基因：'mer'基因和Hg（2+）抗性

Using Ethidium To Probe Nonequilibrium States of DNA Condensed for Gene Delivery

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