Highly Selective Ligand Binding by Methylophilus methylotrophus Cytochrome c′′

Pedro O. Quintas Teresa Catarino Smilja Todorovic and David L. Turner

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Highly Selective Ligand Binding by Methylophilus methylotrophus Cytochrome c′′

机译：甲基嗜甲基菌细胞色素c''的高选择性配体结合

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Cytochrome c′′ (cyt c′′) from Methylophilus methylotrophus is unusual insofar as the heme has two axial histidine ligands in the oxidized form but one is detached when the protein is reduced. Despite cyt c′′ having an axial site available for binding small ligands, we show here that only NO binds readily to the ferrous cyt c′′. Binding of CO, as well as CN–, on the other hand requires considerable structural reorganization, or reduction of the disulfide bridge close to the heme. Standard free energies for the binding of NO and CO reveal high selectivity of the ferrous cyt c′′ for NO, indicating its putative physiological role. In this work, we characterize in detail the kinetics of NO binding and the structural features of the Fe2+–NO adduct by stopped-flow and resonance Raman spectroscopy, respectively.

机译：甲基嗜甲基菌的细胞色素c''（cyt c''）是不寻常的，因为血红素有两个轴向的组氨酸配体呈氧化形式，但是当蛋白质被还原时一个被分离。尽管cyt c''具有可用于结合小的配体的轴向位点，但在此我们显示只有NO容易与亚铁cyt c''结合。另一方面，CO和CN–的结合需要相当大的结构重组，或还原血红素附近的二硫键。用于结合NO和CO的标准自由能表明，cyt亚铁胞质c''对NO的选择性高，表明其推测的生理作用。在这项工作中，我们分别通过停止流和共振拉曼光谱详细描述了NO结合的动力学和Fe2 + -NO加合物的结构特征。

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《Biochemisty》 |2011年第25期|p.5624-5632|共9页
作者
Pedro O. Quintas Teresa Catarino Smilja Todorovic and David L. Turner;
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Instituto de Tecnologia Qumica e Biolgica, Universidade Nova de Lisboa, Av. da Repblica, 2780-157 Oeiras, PortugalDepartamento de Qumica, Faculdade de Cincias e Tecnologia, FCT, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal;

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1. Highly Selective Ligand Binding by Methylophilus methylotrophus Cytochrome c '' [J] . Quintas PO, Catarno T, Todorovic S, Biochemistry . 2011,第25期

机译：甲基嗜甲基菌细胞色素c的高度选择性配体结合''
2. Solution structure of Methylophilus methylotrophus cytochrome c '': Insights into the structural basis of haem-ligand detachment [J] . Brennan L., Fareleira P., Santos H., Journal of Molecular Biology . 2001,第2期

机译：甲基嗜甲基菌细胞色素c的溶液结构''：深入了解血红素配体脱离的结构基础
3. Cloning and sequence analysis of the gene encoding Methylophilus methylotrophus cytochrome c", a unique protein with a perpendicular orientation of the histidinyl ligands. [J] . Price NJ, Ribeiro G, Costa JV, Biochimica et biophysica acta: international journal of biochemistry and biophysics . 1999,第1期

机译：编码嗜甲基菌甲基嗜甲基细胞色素c”的基因的克隆和序列分析，这种独特的蛋白质具有与组氨酸配体垂直的方向。
4. Discovery, sequencing and synthesis optimization of a highly selective ligand binding an orphan adrenoreceptor subtype in the green mamba venom [C] . Loic Quinton, Celine Rouget, Gilles Mourier, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：高精度配体的发现，测序和合成优化在绿色曼巴纳毒液中结合孤儿肾上腺素亚型的高度选择性配体结合
5. A tale of two enzymes: Identification of an unknown ligand bound to cytochrome P450 2A13 and understanding substrate selectivity of cytochrome P450 2E1. [D] . Porubsky, Patrick R. 2009

机译：两种酶的故事：鉴定与细胞色素P450 2A13结合的未知配体，并了解细胞色素P450 2E1的底物选择性。
6. Cytochrome c isolated from Methylophilus methylotrophus. An example of bis-histidine-co-ordinated Fe3+ haem with near-perpendicular orientation of the ligands. [O] . M J Berry, S J George, A J Thomson, 1990

机译：从甲基嗜甲基菌中分离出的细胞色素c。双组氨酸配位的Fe3 +血红素的例子配体的取向接近垂直。
7. Cloning and sequence analysis of the gene encoding Methylophilus methylotrophus cytochrome c″, a unique protein with a perpendicular orientation of the histidinyl ligands [O] . Price Nicholas J., Vijgenboom Erik, Ribeiro Graça, 1999

机译：甲基嗜甲基细胞色素c”的编码基因的克隆和序列分析
8. Time-resolved infrared studies of the dynamics of ligand binding to cytochrome c oxidase. [R] . Dyer, R. B., Peterson, K. A., Stoutland, P. O., 1991

机译：时间分辨红外研究配体与细胞色素c氧化酶结合的动力学。

Highly Selective Ligand Binding by Methylophilus methylotrophus Cytochrome c′′

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