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首页> 外文期刊>Biochemistry >SufD and SufC ATPase Activity Are Required for Iron Acquisition during in Vivo Fe-S Cluster Formation on SufB
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SufD and SufC ATPase Activity Are Required for Iron Acquisition during in Vivo Fe-S Cluster Formation on SufB

机译：SufB上体内Fe-S团簇形成过程中铁的采集需要SufD和SufC ATPase活性

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摘要

In vivo biogenesis of Fe-S cluster cofactors requires complex biosynthetic machinery to limitnrelease of iron and sulfide, to protect the Fe-S cluster from oxidation, and to target the Fe-S cluster to thencorrect apoenzyme. The SufABCDSE pathway for Fe-S cluster assembly in Escherichia coli accomplishesnthese tasks under iron starvation and oxidative stress conditions that disrupt Fe-S cluster metabolism.nAlthough SufB, SufC, and SufDare all required for in vivo Suf function, their exact roles are unclear.Here wenshow that SufB, SufC, and SufD, coexpressed with the SufS-SufE sulfur transfer pair, purify as two distinctncomplexes (SufBC2Dand SufB2C2) that contain Fe-S clusters and FADH2. These studies also show that SufCnand SufDare required for in vivo Fe-S cluster formation on SufB. Furthermore, while SufDis dispensable fornin vivo sulfur transfer, it is absolutely required for in vivo iron acquisition. Finally,we demonstrate for the firstntime that the ATPase activity of SufC is necessary for in vivo iron acquisition during Fe-S cluster assembly.
机译：Fe-S簇辅因子的体内生物发生需要复杂的生物合成机制，以限制铁和硫化物的释放，保护Fe-S簇免受氧化，并靶向Fe-S簇以纠正脱辅基酶。大肠杆菌中Fe-S簇组装的SufABCDSE途径在铁饥饿和氧化应激条件下完成了这些任务，这些条件破坏了Fe-S簇的代谢。尽管SufB，SufC和SufD都是体内Suf功能所必需的，但它们的确切作用尚不清楚。在这里，文中显示SufB，SufC和SufD与SufS-SufE硫转移对共表达，可以纯化为两个不同的复合物（SufBC2D和SufB2C2），其中包含Fe-S簇和FADH2。这些研究还表明，SufCn和SufD是SufB上体内Fe-S团簇形成所必需的。此外，尽管SufDis可用于体内进行硫转移，但对于体内铁的获取绝对是必需的。最后，我们首次证明了SufC的ATPase活性对于Fe-S团簇组装过程中体内铁的获取是必需的。

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