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首页> 外文期刊>Biochemistry >Biochemical and Physiological Characterization of a BLUF Protein−EAL Protein Complex Involved in Blue Light-Dependent Degradation of Cyclic Diguanylate in the Purple Bacterium Rhodopseudomonas palustris
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Biochemical and Physiological Characterization of a BLUF Protein−EAL Protein Complex Involved in Blue Light-Dependent Degradation of Cyclic Diguanylate in the Purple Bacterium Rhodopseudomonas palustris

机译：BLUF蛋白-EAL蛋白复合物的生化和生理学表征涉及紫细菌Rhodopseudomonas palustris中的环二胍的蓝光依赖性降解。

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摘要

Organisms adapt their physiologies in response to the quality and quantity of environmentalnlight. Members of a recently identified photoreceptor protein family, BLUF domain proteins, use a flavinnchromophore to sense blue light. Herein, we report that PapB, which contains a BLUF domain, controls thenbiofilm formation of the purple photosynthetic bacterium Rhodopseudomonas palustris. Purified PapBnundergoes a typical BLUF-type photocycle, and light-excited PapB enhances the phosphodiesterase activitynof the EAL domain protein, PapA, which degrades the second messenger, cyclic dimeric GMP (c-di-GMP).nPapB directly interacts with PapA in vitro in a light-independent manner and induces a conformationalnchange in the preformed PapA-PapB complex. A PapA-PapB docking simulation, as well as a site-directednmutagenesis study, identified amino acids partially responsible for the interaction between the PapA EALndomain and the two C-terminal R-helices of the PapB BLUF domain. Thus, the conformational change,nwhich involves the C-terminal R-helices, transfers the flavin-sensed blue light signal to PapA.Deletion of papBnin R. palustris enhances biofilm formation under high-intensity blue light conditions, indicating that PapBnfunctions as a blue light sensor, which negatively regulates biofilm formation. These results demonstrate thatnR. palustris can control biofilm formation via a blue light-dependent modulation of its c-di-GMP level by thenBLUF domain protein, PapB.
机译：生物适应环境光的质量和数量来适应其生理。最近发现的感光蛋白家族BLUF域蛋白的成员使用黄素发色团来感测蓝光。在本文中，我们报道PapB，其中包含一个BLUF域，控制紫色光合细菌大红假单胞菌的生物膜形成。纯化的PapB经历典型的BLUF型光循环，光激发的PapB增强EAL域蛋白PapA的磷酸二酯酶活性，该蛋白降解第二个信使，环状二聚体GMP（c-di-GMP）.nPapB在体外与PapA直接相互作用以不依赖光的方式，并在预先形成的PapA-PapB复合物中诱导构象变化。 PapA-PapB对接模拟以及定点诱变研究确定了部分负责PapA EALn结构域与PapB BLUF结构域的两个C端R螺旋之间相互作用的氨基酸。因此，构象变化（涉及C端R螺旋）将黄素敏感的蓝光信号转移到PapA。papBninR. palustris的缺失增强了高强度蓝光条件下生物膜的形成，表明PapBn起着蓝色的作用。光传感器，对生物膜的形成产生负面影响。这些结果证明了nR。 palustris可以通过thenBLUF域蛋白PapB对其c-di-GMP水平进行蓝光依赖性调节来控制生物膜的形成。

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  • 来源
    《Biochemistry》 |2010年第50期|p.10647-10655|共9页
  • 作者

    Takuya Kanazawa Shukun Ren Mikika Maekawa Koji Hasegawa Fumio Arisaka Mamoru Hyodo Yoshihiro Hayakawa Hiroyuki Ohta and Shinji Masuda;

  • 作者单位

    ‡Graduate School of Bioscience and Biotechnology, and §Center for Biological Resources and Informatics, Tokyo Institute ofTechnology, Yokohama 226-8501, Japan,) Advancesoft Corporation, Akasaka, Tokyo 107-0052, Japan,^Graduate School ofPharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan,@Faculty of Engineering, Aichi Institute of Technology,Toyota 470-0392, Japan, and #PRESTO, Japan Science and Technology Agency, Saitama 332-0012, Japan;

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